| Purpose:Skin aging not only influences our physiognomy, but also relates to many dermatogic disease in etiology, thus, anti-aging or ageing deferring becomes more and more popular in medical science and aesthetic. With long application and observable curative effect, it has been studied that some traditional Chinese medicines are showing their superiority in anti-aging. It is approved by scholar both demestic and abrod that some Chinese medicine with curative effect of invigorating vital energy, like the decoction of four mild drugs (DFMD) and radix astragali, can increase the activity of super oxidation dismutase (SOD) and GSH—Px caused by D-galactose or O3 in the brains, livers, bloods and whatsoever of mice, drosophila and counterparts, meanwhile decrease the percentage of malonaldehyde(MDA ), the three index mentioned above are the index showing the free radical and can further improve the curative effect of anti-oxidation of invigorate vital energy Chinese traditional medicine. Besides, the decoction of four mild drugs (DFMD) can increase the percetage of hydroxyproline and I-collagen in the skins of the rats in aging rat model. The decoction of four mild drugs (DFMD)is a sort of classical invigorate basic dose found in Tai Ping Hui Min He Ji Ju Therapy and is with wide application in clinic. In conclusion, it is with generalization value to make further study of the anti-aging effect and application of The decoction of(DFMD). Decoction of Four Mild Drug is applied to plastic surgery after lifting as a postoperative recovery medication.It can improve skin antioxyen capacity,increase collagen content and slow down the rate of skin aging.It can be a long time to maintain the lifting effect. It will become a good assistant drug of lifting.Methodology:20 three-months SD rats, sexless, weight 200-250g, divided into 5 groups at random- Chinese medicine control group (5 rats), model control group (5 rats), positive control group (5 rats) and normal control group (5 rats). For Chinese medicine control group, the cervicals and backs of the rats are hypodermic injected with 10% D-galactose liquor(1000mg/kg)and fed with Decoction of Four Mild Drugs (DFMD) 2g/ml,48g/kg per day. For model control group, the rats are hypodermic injected with same D-galactose liquor per day. For positive control group, the rats are hypodermic injected with same D-galactose liquor and fed with Vitamin E 200mg per day. For normal control, the cervicals and backs of the rats are injected with NS with same quantity 10% D-galactose liquor used by other groups. Six weeks later, the SOD activeness, MDA content and hydroxyproline (HYP) content of skins and the thickness and status of epidermis and dermis was to be observed, and I-collagen ( ICOL ) was to be observed by way of immunohistochemistry.Result : The cervical and back of the rats are hypodermic injected with D-galactose(1000mg/kg·d), six weeks later, the MDA content in the skin of model control group rats have increased more evidently than other groups, but the SOD activeness is slower than others'. As the decoction of four mild drugs (DFMD) can lower the MDA content of radical metabolite, the content of the Chinese medicine control group is less that normal control group's and model control group's; but the SOD activeness is quicker than normal control group's and model control group's and there is no remarkable difference with the positive control group's. The hydroxyproline (HYP) content of Chinese medicine control group is much more than others', when light microscope is used, the model control group-- epidermis and dermis become thinner, fibroblasts are reducing, the alignment of skin accessory structure becomes loose; the area of I-collagen( ICOL) is minimizing through immuneohistochemistry; the skin and I-collagen(ICOL)of Chinese medicine control group and positive control group content are thicker/more than those of model control group and the thickness difference and I-collagen difference between the Chinese medicine control group and positive control group are observably.Conclusion:matched group。With great anti-oxidation ability, it is concluded that increasing/decreasing Chinese - Decoction of Four Mild Drugs (DFMD) can improve the SOD activeness of skin, in way of minimizing the damage to the skin caused by the peroxidate accumulation, esp. it can provide better protection for the collagen in the skin than the positive control group's. |
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