The Effect And Mechanism Of Radiation To Reduce Restenosis After Transurethral Resection Of The Prostate

Benign prostatic hyperplasia(BPH) is a common disease of old men. transurethral resection of the prostate is a new technique to cure BPH patients in recent years which can provide much advantage such as little hurt and wide adaptability, but about fifteen percent to twenty percent patients who underwent TURP can recur after one to ten years. Very little is known about how to reduce restenosis after TURP. In the study, we estimated the effect and safety of P irradiation to reduce restenosis after transurethral resection of the prostate in clinical application and observed the effect of ft irradiation to induce apoptosis of prostatic cells by irradiating prostatic tissues or by cultivating prostatic cells in vitro. We also investigated the molecular mechanism of radiation to reduce restenosis after transurethral resection of the prostate by detecting the expression of apoptosis-correlating genes in prostate for the wide use of radiation in transurethral resection of the prostate. I. The effect and safety of B irradiation to reduce restenosis after transurethral resection of the prostateBPH patients were divided into three groups, group A in which patients only received ft irradiation to treat BPH. group B in which patients received ftirradiation to reduce restenosis before transurethral resection of the prostate, group C in which patients only received transurethral resection of the prostate. The irradiation dose distribution and safety of irradiation in clinical application was studied. Characteristics of dosimetry of irradiation were determined using nuclear detection techniques in body-phantom. The distribution of the surface dose rate had been measured using lithium fluoride thermoluminescent dosimeter during the treatment of the patients. We found that the absorbed doses of B-ray were decreased with the increased distances and varied markedly in the range of thirteen millimeters, no obvious difference was found in the results of surface dose rate of bremsstrahlung, the absorbed doses of doctors and patients were much lower than that of the nation limits. By investigating the effect of B-rays on BPH. we found that in group A the syndrome alleviated remarkably, the MFR increased, PVR and IPSS decreased remarkably. There was significant difference on MFR. PVR, I-PSS and volume of prostate gland before and after transurethral resection of the prostate. In the first year after transurethral resection of the prostate all of the indexes in group B stabilized but the MFR decreased and PVR and IPSS increased in group C. It suggested that prostatic hyperplasia recurred in different degrees after operation. The observation of the histological changes showed that B-rays can make the prostatic tissues wither gradually and the shape of nucleus of prostatic cells was abnormal. The detection of electron-microscopy were that in radiation group the stroma and the mitochondria swelled and vacuoles appeared in nucleus, nucleus were larger, the membrane of nucleus broadened, chromatin condensed and apoptotic bodies came into being. It confirmed that irradiation can induce apoptosis of prostate to reduce reslenosis.2. The mechanism of radiation to reduce restenosis after Iransurelhral resection of the prostateIn the study. BPH patients received P irradiation from one to fifteen days before transurethral resection of the prostate and the mechanism of radiation to reduce restenosis was investigated. The protein expression of bcl-2 and bax and TGF- P i and bFGF in prostatic tissues was examined by immunohistochemical and Western blot method and at the same time the mRNA expression was examined by in situ hibridization method. The result showed that the expression of bcl-2 in control group was higher than that in normal group, the expression of bcl-2 in prostate treated with P irradiation decreased. The protein expression of bax in epithelia in normal group was higher than that in control group, and the expression of bcl-2 protein in epithelia and stroma in control group was higher than that in normal group. Treated with...