Long-term Consequences Of Renal Ischaemia-reperfusion Injury In The Rat And Intervention

Renal ischaemia-reperfusion injury is evitable pathophysiological process inkidney transplantation. There are still existing in acute renal injury, cardiopulmonaryresuscitation, cardiopulmonary bypass and operation with renal artery occlusion.Although much attention has been focused on events occurring during the ischemicevent or in the early recovery phase, the long-term effects of ischemic injury havereceived little study. Recently, some studies show that ischaemia-reperfusion injurynot only cause acute injury but also lead to long-term renal function deterioration. Weexplore the consequences of long-term injury after ischaemia-reperfusion injury inrat and the effect of astragaloside IV on it. In part 1, we observe whether or notastragaloside IV is effective on preventing renal injury from ischaemia-reperfusioninjury, and choose a most effective dose for part 2 if it is . we study the long-termeffects of ischaemia-reperfusion injury inrats and the effect of astragaloside IV on itduring part 2. ABSTRACTPart 1:Administration of astragaloside IV prevents renal ischaemia-reperfusioninjury in ratsPurpose: To explore the effect of astrgaloside Ⅳ on renal ischaemia-reperfusioninjury in rats.Methods: 30 Sprague-Dawley male rats (240-260g) were randomized into Modelgroup, sham group and three different doses of astrgaloside Ⅳ groups (High group,Medium group and Low group). Animals in High group, Medium group and Lowgroup was given 10% astrgalosideⅣ, 5% astrgaloside Ⅳand 1% astrgaloside Ⅳ 2mlseparately by daily gavage 2h before operation for three days, other groups treatedwith equal dose of normal saline. After right kidney was removed, the renal artery ofthe left kidney were occluded with a vascular clamp for 60 minutes. Serum creatinine(Cr) and renal tissue superoxide dismutase(SOD) activity and tumour necrosisfactor-alpha (TNF-a) was measured; Partial renal tissues were removed for 3肾脏缺血再灌注损伤的远期改变及干预pathological examination 24h after reperfusion.Results: We observed sham group showed few renal pathological changes, High group decreases renal pathological injury compared with that of Model group andother astrgaloside Ⅳ groups, while improves SOD activity (98.25±10.35NU/gtissue) and inhibits serum Cr (24.93±2.49μmol/L)and TNF-a(3.34±0.29ng / gtissue) in renal tissue significantly compared with that of Model group(renal tissueSOD73.08±7.30 NU/g tissue,Cr 34.93 ±3.35μmol/L,renal tissue TNF-a7.28±0.21ng / g tissue)(P<0.05). there were no significantly difference among Modelgroup and other astrgaloside Ⅳ groups(P>0.05).Conclusions: These results suggest that 10%astrgaloside Ⅳ markedly amelioratedrenal ischaemia-reperfusion injury, possibly by improving SOD activity and inhibitingTNF-a in renal tissue.