| Section 1:The quantitative study of testicular spermatogenous function in rats with testicular torsion/detorsionObjective: The testicular torsion is also called torsion of spermatic cord.It is one of urological emergency.The torsion of spermatic cord means that spermatic cord rotates around its axis of ordinate.In consequence,it causes obstacle of the blood circulation of spermatic cord and leads to testicular ischemia or even necrosis.The morbidity of the disease reaches up to 1 of 4000 men by the age of 25 years old.As a result,the disease isn't seldom seen.In clinical treatment,patients with testicular torsion for short time are performed testicular reduction and orchidopexy to recover testicular blood supply and avoid testicular infarction.However,have testes change in spermatogenous function? For this purpose,we carried out following study. We made the rat model with testicular torsion/detorsion.Testicular spermatogenous function was evaluated scientifically by analysing testicular weight as well as testicular convoluted seminiferous tubule in quantity. Methods:20 young adult male Sprague-Dawley rats with weight from 300 to 320grams were shopped from Shanghai experimental animal center of Chinese scientific academy. 20 rats were divided into two groups at random. 10 were in control group of sham operation and the other 10 were in testicular torsive group.The model of rat testicular torsion/detorsion was established in terms of Ozkan's method.In control group,rat left scrotum was incised and left testis was dissociated and exposed ,but it hadn't been performed torsion.A 11/0 atraumatic silk suture was placed through the tunica albuginea. In torsive group,left testis was rotated 720° in a counterclockwise direction and maintained in the torsive position by fixing testis to scrotum with 11/0 atraumatic silk suture.After two hours,the torsive testis was detorsed and hadn't infarction.Two groups rats had been fed for 7 days at the same condition.The 7th day after operation,experimental rats were anesthetized and their scrotums were reincised.We found that there wasn't obvious difference in size between left and right testes in rats of control group.However,the sizes of left testes were apparent less than those of right testes in rats of torsive group.The left and right testes were excised and weighed in electronic scales.Then testicular tissues with thickness of 0.2-0.3 CM were cut from whole testes and fixed in Bouin's solution.At last,fixed tissues were made into paraffin sections by a series of techniques.We assessed testicular spermatogenous function by Makler score. Under microscope, 10 roundest convoluted seminiferous tubular broken faces were observed in every section. We tested respectively inside diameter of convoluted seminiferous tubule(D), thickness of peritubular membrane(M) , germ cell's layer number in tubular wall(P) and mature degree of germ cell(S).The score of every item varies from 0 to 5.The fullmark of four items is 20.The average mark (also called Makler Score) was calculated according to marking result. The Makler Score is the more higher,testicular spermatogenous function is the more better.Results:The weights of left and right testes were 1.653±0.088 grams and 1.662±0.093 grams respectively in rats of control group.By comparison,there wasn't significant difference(P>0.50 ).The weights of left testes were obvious lighter than those of right testes in rats of torsive group(0.915±0.148 grams versus 1.704±0.096 grams,P<0.001).At the same time,weights of left testes in rats of torsive group were also significant lighter than those of left testes in rats of control group(0.915±0.148 grams versus 1.653±0.088 grams, P<0.001).There wasn't significant difference in right testes between torsive and control groups(1.704±0.096 grams versus 1.662±0.093 grams, P>0.20).The left testicular D, M, P, S. Makler Score in turn were 234.85±23.42 um,1.980±0.598um,7.01±1.60 layers,4.81±0.39 , 19.81±0.39 in rats of control group;the right testicular D, M, P, S, Makler Score in turn were 235.45±21.53 um... |
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