| Objective: To investigate rats unilateral testicular torsion resulting in ipsilateral and contralateral testicular germ cell apoptosis and testicular tissue catalase and glutathione peroxidase activity changes. Discussion on unilateral testicular torsion / detorsion the protective mechanism of astragalus injection and decreased reproductive capacity after its reperfusion injury in rats .Methods:1 Animal groups: fouty health adult Wistar rats with weight from 210 to 290 grams were divided randomly into four groups.Sham operation group (group A,n=10), Torsion/detorsion group (group B,n=10),single astragalus plus detorsion group ( group C n=10),Successive astragalus plus detorsion group ( group D,n=10).2 Model: Testicular torsion/detorsion model was established in terms of Turner methods. All surgical procedures were performed with the rats under intraperitoneal 2% pentobarbital sodium (5mg/Kg) anesthesia. In the sham operation group, the testes were brought throught the incision and replaced , and a silk suture placed through the tunica albuginea. Except the sham operation group,the rats were subjected to the left unilateral testicular torsion (720 rotation in the clockwise direction). Torsion and sham operations were performed throught standard ilioinguinal incisions.The left testicle was fixed to the scrotum by a silk suture placed throught the tunica albuginea to prevent the spontaneous detorsion. After all the surgical procedures and application, the incision was closed with silk suture, After 6h with the same method again anesthesia group B, C, D, after the dismantling of its three groups wound sutured, left testicular torsion reduction and fixation. Astragalus (3g/kg )was injected intraperitoneally 30 min before detorsion once in group C, In group D except astragalus (3g/kg) was injected intraperitoneally 30 min before detorsion once , astragalus was successive injected 3 days. All rats were fed under the same conditions for 7days and sacrificed, bilateral orchidectomy was performed All organ were washed two times solution, labeled and stored in a deep freeze(-30°)until processing. Both testes were cut, after washing twice with cold saline vertically divided into two parts, a part was homogenised, the other parts were made part of paraffin sections.3 Germ cell apoptosis detection : In situ deoxynucleotidyl transferase - mediated dUTP nick end labeling (TUNEL) Apop–totic cells apoptotic nuclei brown color. Apoptosis index (AI) to calculate each slice select five high-power fields (×200), counting 100 cells and achieving positive cells. 4 Catalase (catalase. CAT) and glutathione peroxidase (GSH - Px) activity assay determination : CAT activity was determined according visual light methods. GSH– Px activity was measured by the method of paglia.5 Statistical analysis :All data were statistically analyzed by using X±s.Every groups were compared by using analysis of variance to compare samples with SPSS12.0 software for statistical analysis. Testing standards atα= 0.05.Results:1 Testicular germ cell apoptosis: AI of group A germ cell apopt- osis (sham control group) and the reverse side of testi–cular AI of group B, C, D was a significant difference (P<0.05). Group B (testicular torsion / detorsion) torsion testicular AI was significantly higher than that in group C (testicular torsion / detorsion + astragalus single intraperitoneal injection ) and group D (testicular torsion / detorsion and astragalus continuous intraperitoneal injection ). AI of group C and group D torsion testes was significant differences (p <0.05) . AI of group C and group D contralateral testes compared with group B was significant differences (p <0.05) , AI of group C and group D contralateral testis compared with the contralateral testicular AI signi ficant difference (p <0.05), AI of group C and group D contralateral testicular significant differences in the two groups (p<0.05).2 CAT and GSH - Px activity : In the reverse side of testicular, about GSH - Px and CAT ,there was a significant difference among group A,B,C,D (p <0.05).In group B, reverse side of testicular CAT and GSH - Px activity was significantly lower than that in group C and group D (P <0.05), reverse side of testicular CAT and GSH - Px activity between group C and group D was significantly difference.In group B, CAT and GSH - Px of contralateral testes significantly lower than that other groups, CAT and GSH - Px of contralateral testes between C and D groups no significant difference (p>0.05).Conclusion:1 Unilateral testicular torsion can induce ipsilateral and contral- ateral testicular germ cell apoptosis significantly increased. so reproductive capability decreased.2 Unilateral testicular torsion / detorsion result in testicular tiss- ue catalase and glutathione peroxidase activity decreased.3 Astragalus injection could significantly reduce the ischemia a- nd reperfusion injury caused by testicular torsion / detorsion . Bilateral testicular germ cell apoptosis reduced, thereby protecting rat fertility, Continuous application of astragalus injection is better than a single application.
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