| This research is a different and similar to make use of histamine-skin experiments to understand the Chinese herbal medicine antihistamine principle, contrast western medicine antihistamine function. Research method, result 1. Clinical research1.1 Research method:Draft health volunteer. Establish small-blue-dragon soup group, ma-huang soup group, loratadine group with placebo group. Observe the object take the small-blue-dragon soup respectively with the ma-huang soup, loratadine 10 mg or placebo ones. Histamine skin tests (histamine concentrations 20. 0 , 7. 3 , 2. 7 , 1. 0 mmol/L ) were performed for each one before dosing and 2, 5, and 24 hours after dosing. After 15 minutes measure wheal and flare areas, draw the AUDRC ( area under dose- response curve, AUDRC) , using trapezoid method calculation AUDRC for each subject, and compared. All AUDRC were analysis in one-way ANOVA.1.2 Result:1. 2. 1 The wheal and flare area compared with itself after dose and before doseThere was no significantly differed in small-blue-dragon soup and ma-huangsoup( P>0. 05), in loratadine shows a highly significant treatmenteffecy( P<0. 05).1.2.2 The wheal and flare area of small-blue-dragon soup, ma-huang soup, theloratadine compared with placebo groupNo significantly differde in small-blue-dragon soup , ma-huang soup , loratadine , placebo before dose( P>0. 05), no significantly differed insmall-blue-dragon soup, ma-huang soup compared with placebo after dose 2,5,24 hours( P>0. 05), the analysis of the loratadine and placebo shows a highly significant treatment effect. ( P<0.01)1. 2. 3 AUDRC The dose-response curves was no significantl differed in small-blue-dragon soup,ma-huang and placebo. The AUDRC of small-blue-dragon soup, ma-huang, loratadine and placebo before dose was no signinificantly differed. ( P>0. 05)The AUDRC of small-blue-dragon soup,ma-huang soup compared with placebo was no significantly( P>0. 05). The AUDRC of loratadine compared with with placebo shows a highly significant. ( PKO. 01)2. Experiment research2. 1 Material with methodUsing the small-male-rat in 8 weeks of C57BL6 system , build the model according to CHAOCANG method that build molder with ovumalbumin , using 2ml N. S contain 400mg ovumalbuminto dripping nostrils , once in a day, totally 4 times, observing the nose symptoms of small rat in firstly 10 minutes after every dripping nose, in the second days after the last dripping nose, confirm the histamine threshold value with the different density histamine dripping nose. ( firstly 10 minutes scratch nose >20 times ) 2.2 Result 2. 2. 1 The influence of the mice nose symptoms by ma-huang soupsThe small mice nose symptom of the model control groups shows the trend was aggravated, giving medicine group, prevent giving medicine group can alleviate the nose symptom , compare with the giving medicine and the model control group shows highly difference , p<0. 01.2. 2. 2 The influence of histamine threshold by ma-huang soups The histamine threshold of giving medicine group and prevent giving medicine group is higher than control group , it shows that giving medicine can increase histamine threshold. Discussion 1. The anti-allergic function of the small-blue-dragon soup and the ma-huang soupThis research we observe their anti-histamine principle with ma-huang for main medicine of the small-blue-dragon soup and the ma-huang soup in representative of the Chinese herbal medicine .The small-blue-dragon soup usedfor the treatment of the asthma firstly, then used for the treatment of allergic rhinitis afterwards and get affirmative of domestic and international .Someone made the research about the small-blue-dragon anti-histamine pharmaco, such as Da-jiu-bao reports the small-blue-dragon soup can repress the exists rate of acidophilic-cell obviously , can repress obviously ovum-albumin, person immunity-globulin and persons secrete type immunity globulin takes off grain etc..Ma-huang soup is used to treat asthma, also having to be used for the treatment allergic rhinitis, but the anti-histamine pharmaco did not see, therefore we made the anti-allergic experiment about ma-huang soup. Seeing from experiment result, ma-huang soup contain the function of ameliorated the nose allergic symptom in certainly, can increase the nose velum to bear to suffer of the histamine, this may be the main reason of its alleviative nose symptom.2. Make use of the histamine-skin-test to establish the efficacy of medicine dynamics modelThis experiment to adopt the histamine skin titration tests, investigating the relation of histamine density on the different density level and wheal( or flare), can increase the accuracy. Adoption AUDRC as parameter that size, considered 4 different densities at the same time to cause the wheal or flare responses, comparing with one density, is more accurate and intui- tionistic to efficacy of medicine dynamics model of the analysis. Take loratadine after he settled, the dose-response curve descend moving, the AUDRC let up, therefore loratadine' s anti-histamine function effect was rising quickly, and the function keeps on long time and is strong, agreeing with the dynamics characteristic of that medicine.3. Make use of histamine-skin-test contrast the different and similar of anti-histamine function about the Chinese medicine and the Western medicineThe anti-histamine function of the medicine may have three kinds of paths: (1) directly repress histamine. (2) to fight with histamine to contest the histamine accept-body, indirectly repress histamine, the anti- histamine medicine of the clinical usage, such as loratadine is belong to this kind. (3) repress the fatty-big cell taking off the grain function. Combined our the research that mentioned, the small-blue-dragon soup and the ma-huang soup are confirmedstronger anti-allergic and anti-histamine function in clinic and experiment. In this experiment, taking the small-blue-dragon soup and the ma-huang soup in front and back the wheal and flare area and AUDRC did not show the obvious change, the small-blue-dragon soup and ma-huang soup compare with placebo after taking the wheal and flare area and AUDRC did not show obvious change. Because the histamine in our experiment is a out-source that injected into , but the histamine caused symptom in the pathologic process in allergic rhinitis is in-source histamine that be secreted by fatty-big cell taken off grain explain. That show the small -blue-dragon soup and the ma-huang soup can't repress histamine directly .The local Lee' s report shows a small -blue-dragon soup represses the fatty- big cell of big rat belly cavity taking off grain and secreted histamine .Combine the abroad concerning the main pathologic function of the small-blue-dragon soup repressed the big rat belly cavity fatty-big cell to leave , we can predict the small-blue- dragon soup and the ma-huang soup's anti-histamine mechanism is primarily to repress the fatty-big cell taking off the grain, reducing histamine secrete. So we predict the different and similar of the Chinese medicine and the western medicine's anti-histamine function:the same is the both can't repress histamine directly, the different is western medicine to fight with histamine to contest histamine accept body, indirectly repress histamine, the out-source and in-souce histamine can repressed . But the Chinese herbal medicine is to repress the fatty-big cell taking off the grain function, reducing histamine secrete.We should point out that the Chinese herbal medicine is composed by the compound prescribe, implying various valid compositions, for this reason Chinese herbal medicine curing this disease is very complex, is pass several links to rise function, anti-histamine is an important link among them. Cone I us i on:1. According to the restraint function in histamine-skin-test of the anti-histamine medicine can establish the efficacy of medicine dynamics model, the AUDRC can be used as an index sign of the comparison anti-histamine medicine efficacy of medicine dynamics activity.2. Take the small-blue-dragon soup and the ma-huang soup in front and back the wheal and flare area and AUDRC did not show the obvious change, explaining the...
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