Expression Of Th1/Th2 Types Cytokines & Transcription Factor T-bet MRNA In PBMC Of The Patients With Alopecia Areata And Its Regulation By The Traditional Chinese Herbal Medicines

Objectives First to investigate the relationship between expression of Thl/Th2 types cytokines(IFN- γ, IL-12/IL-10) & transcription factor T-bet mRNA in PBMC of the alopecia areata patients with symptoms of liver-kiney deficiency and TCM symptoms; Second to study the role of expression of Thl/Th2 types cytokines & transcription factor T-bet mRNA in PBMC in the pathogenesis of alopecia areata and its regulation by Procyanidins(PC) and Astraglus polysaccharide(APS).Methods Apply RT-PCR technique to detect the mRNA expression of Thl/Th2 types cytokines & transcription factor T-bet in PBMC of the patients with alopecia areata by stimulation with phytohemagglutinin(PHA) and PHA plus PC/APS respectively.Results The mRNA expression of Thl type cytokines in PHA-stimulated PBMC of the patients with localized alopecia areata were increased slightly compared with healthy controls, the expression of transcription factor T-bet mRNA markedly elevated with the healthy controls (p<0. 05) , on the contrary, the mRNA expression of Th2 type cytokines (IL-10)lower significantly than healthy controls(P<0. 05). However, the mRNA expression of both Thltype cytokines & transcription factor T-bet in PHA-stimulated PBMC of the patients with extensive alopecia areata were markedly increased compared with healthy controls(P<0. 01)and localized form(P<0. 05), but the mRNA expression of Th2 type cytokines (IL-10)were lower significantly than healthy...