| ObjectiveTo evaluate the improve function of the Gu kang on the quality of the bone in the PMOP through the animal's experiment and the clinical research.MethodThe animal's experiment :72 femal 6-month-old SD rats were randomized into six groups of 12 each: the shame-operating group, ovariotomized (QVX)group, E2 treated group ,Gu -kang of high dosage group, Gu kang of middle dosage group, Gu kang of low dosage group, all of rats of the later five groups were ovariotomized. After the model of PMOP was established, the BMD> histomorphometric analysis and the biomethanics of the bone were measured.The clinical research: 70 PMOP patients(2003. 03-2005. 01) were randomly divided into Gu kang group and Alendronate sodium tablets group. Both groups take medicines for 6 months. After six months treatment, we observe BMD, E2, ALP, HOP/ Cr, Ca/Cr.ResultsBMD determine: After 3-month treatment with gukang of high and middle dosage, the BMD of the total and of the lumbar was increased remarkably compared with the OVX group, there was significant difference(P<0.05, P<0. 01). The BMD of bilateral hips of the high and middle dosage groups had the tendency of elevation, but there wasn' t statistics significance (P > 0.05). The BMD of the total femur of high dosagegroup , the R0I4 of the high and middle group , the BMD of the R0I1, R0I2, R0I4 of the Sham-operating group were higher than that of the OVX group (P<0.01,0. 05). The BMD of the R0I1, R0I2, R0I4 of the OVX group, Gu kang of the high and middle group were lower than that of the group(P<0. 01,0. 05). The BMD of the total tibia of the Sham-operating, E2 treated group, Gu -kang of high dosage group, Gu kang of middle dosage group were higher than that of the OVX group(P<0. 01, 0. 05), The BMD in R0.I2 of the high dosage group was higher than that of the OVX group(P<0.05). The BMD in R0I1 of Sham-operating, E2 treated group, Gu -kang of high dosage group, Gu kang of middle dosage group were higher than that of the OVX group(P<0. 01,0. 05). The BMD of the total tibia in Gu -kang of high dosage group, the OVX group, E2 treated group, Gu -kang of high dosage group, Gu kang of higher middle low dosage group, R0I1 of the OVX group were lower than that of the Sham-operating group (P<0.01, 0. 05).Histomorphometric analysis: Double in vivo fluorochrome labeling was administered to all rats. At the end of study, the undecalcified longitudinal proximal tibial metaphyseal section was cut and stained with Masson-Goldner Trichrome for bone Histomorphometric analysis. Results: Compared with the Sham group, the percent trabecular area of proximal tibial metaphyseal(-73.96%) and second lumbar vertebra(-35. 84%) of the OVX group reduced markedly because bone resorption excessed bone formation(P<0. 01). After 3-mouth therapy, in proximal tibial metaphyseal, compared with that of the OVX group, the percent trabecular area(%Tb. Ar) was increased(+113.00% and +84.52%) remarkably in rats treated with Gukang of high and middle dosage respectively, trabecular number(Tb. N) increased (+110. 45%, +72. 51% and +69. 77%) obviously in rats treated with Gukang of high, middle and low dosage, osteoclast number per mm(0c. N/mm) and osteoclast number per mm2(0c. N/mm2) were decreased (-51. 49% and -52. 90%) prominently in rats treated with Gu kang of high dosage, there was significant difference(P<0. 05, P<0. 01). A trend of %Tb. Ar in rats which received Gu kang of low dosage, mineral apposition rate (MAR) in rats which received Gu kang of middle and low dosage , bone formation rate/TV(BFR/TV) in rats which received Gu kang of high, middle and low dosage to increase were revealed(P>0. 05). A tendency of trabecular separation(Tb.SP) and bone formation rate/BV (BFR/BV) in rats treated with gukang of high, middleand low dosage, percent labeled perimeter(%L. Pm) and bone formation rate/BS(BFR/BS) in rats treated with Gu kang of high dosage, Oc.N/mm and Oc. N/mm2 in rats treated with Gu kang of middle and low dosage to decrease were uncovered(P>0.05).Biomechanics performances: The third and fourth lumbar vertebra and right femur taken out to measure biomechanics properties. Compared with the Sham group, the biomechanics properties of the lumbar vertebra and the right bilateral proximal femoral of the OVX group worsened significantly (P<0. 01). After 3-month therapy, compared with the OVX groupi the biomechanics ability of resisting press of cancellous bone of the third lumbar vertebra was improved obviously in rats treated with E2, Gu kang of high and middle dosage (P<0. 01). The targets of structure mechanics and material mechanics of the fourth lumbar vertebra was improved obviously in rats treated with E2, Gu kang of high, middle and low dosage. Among the targets, the break loading, modulus of bending elasticity and break strength in the fourth lumbar vertebrae of experimental group' s rats treatment with E2, Gu kang of high and middle dosage were increased remarkably compared with OVX group (P<0. 05). The structural mechanical strength of the right bilateral proximal femoral of experimental group' s rats treated with E2, Gu kang of high, middle and low dosage were improved obviously compared with OVX group (P<0.05, P<0. 01). But every biomechanics targets of the bilateral proximal femoral of experimental group' s rats was lower than that of Sham group(P<0. 05).Clinical research Result: The total effective rate in the two groups was 90.6% and 85.2%. There was no significant difference (P>0. 05). Significant difference was found after treatment about E2 in the treated group(P<0. 01); E2 between the control and treatment group had significant difference (P<0. 01); There was no significant difference about ATP Ca between both groups (P>0. 05). BMD in the both groups had difference (P<0. 05) . There was no significant difference in both groups (P>0. 05).ConclusionVerification was made that the therapy of the Gu kang to the PMOP is a total mode of regulation on the body through the animal's experiment and the clinical research. The Gu kang have double effect on restraining...
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