Experimental Study Of Guanxinkang On Acute Myocardial Ischemia And P53 And Bcl-2 Effect In Rat

Background:Guanxinkang is an effective drug that be summed up by my tutor.The principle of supplementing qi and activating blood is the therapeutic principle which treating CHD(coronary heart disease) of asthenic qi and blood stasis.In this treatise,through animal experiments studies,we can see themechanisms of how Guanxinkang can play an influential role in prevention and treatment of CHD can be deeply probed into. Objectives:To observe the effect of traditional Chinese medicinal herb (Guanxinkang) on acute myocardial ischemia modle rats,and to approach the action and mechanism of preventing coronary heart disease (CHD). Method:Using modern scientific technology to study Guanxinkang, including pharmacy,pharmacology and phathology.Using Diaoxinxuekang capsule as positive control and acute myocardial ischemia modle rats as study object,we can observe the effect of different concentration Guanxinkang on ECG, CK, CK-MB, LDH,and BLOOD VISCOSITY,NO,ET,as well the adjustment of P53,Bcl -2 gene and apoptosis by test methods such as enzyme assay,optics microscope,electric microscope, morphologic nitrate reduction , immunohisto-chemical method. Results:1 Have Taken Ginsengenin Rgl and Re as the index to select the reasonable technology by the use of orthogonal design method.2 Guanxinkang has obvious dose-dependent decrease effect on model rats S-T segment deviation, CK, CK-MB, LDH, ahd the effect is better than Diaoxinxuekang capsule when middle and high dosage.3 The studies show that Guanxinkang infusioi manifest the anti-damage function to acute myocardial ischemia; Guanxirkang has obvious regulation effect on model rats; it can debase ET,increase NO.