The Experimental Study On Protective Effects And Mechanisms Of Extract From Lxeris Of Panax Notoginseng And Ixeris Sonchifolia-hance On Acute Myocardial Ischemia In Rats

Background:Extract fromlxeris of Panax notoginseng and Ixeris sonchifolia-hance (SK) is an effective durg that be summed up by my tutor.The principle of supplementing qi and activating blood is the therapeutiec prineiple Which treating CHD(coronary heart disease) of asthenic qi and blood stasis.In this treatise,through animal experiments studies,we can see themechanisms of how Extract SK can play an influential role in prevention and treatment of CHD can be deeply probed into.objectives:To observe the effect of traditional Chinese medieinal herb(SK) on acute myocardial ischemia modle rats,and to approach the action and mechanism of preventing coronary heart disease(CHD).Method:Using modern scientific technology to study SK,including pharmacy, pharmacology and phathology.Using danshendiwan positive control and acute myocardial ischemia modle rats as study object,we can observe the effect of different concentration SK on ECG,and BL00D VISCOSITY,CK,CK-MB,as well the adjustment of Bcl-2,Caspase-3 gene and apoptosis by test methods such as enzyme assay,opties microscope,electric microscope,morphologic nitrate reduction,immunohisto-chemical method,western bioting.Results:1 SK has obvious dose-dependent decrease effect on model rats S-T segment deviation, CK,CK-MB,and the effect is better than danshendiwan when middle and high dosage.2 The studies show that SK infusion manifest the anti-damage function to acute myocardial ischemia;SK has obvious regulation effect on model rats;it can debase CK, CK-MB.3 The studies show that SK infusion manifest the anti-damage function to acute myocardial ischemia and can protect cardiac muscle cell;SK has obvious improvement effect to micro-structure of showing and ultra micro-structure on model rats;it can debase cardiac muscle organize.and the effect is better than Danshendiwan when middle and high dosage.4 SK infusion may increase or disrupt the porceduer of cell apoptosis by regulating the expression of Bcl-2 gene and Caspase-3 gene,depress or disrupt the over-apoptosis procedure of acute myocardial cells,and the effect is better than Danshendiwan when middle and high dosage.Conclusions:SK have effective anti-myocadrial ischemia function.The mechanism probably relate to inhibiting CK,CK-MB secretion,and depress or disrupt the procedure of cell apoptosis by regulating the expression of Caspase-3 and Bcl-2 gene.The search results shown above suggest that SK can protect cardiac muscle cell and play a significant anti-damage role to myocardial ischemia thorugh a mlltiplechannelled synthetically mechanism.