Mechanism Research Of Wenxia Decoction In The Tumor Inhibition And Inducing Apoptosis In Human Colon Cancer Cells

Objective Using experiment research to inquire deeply into the related mechanism of the effect of wenxia decoction in the tumor inhibition and induce apoptosis of colon cells , anticipating providing new thinking and effective decoctions for clinical use in curing and preventing the Carcinoma of the colon and rectum .Methods on the internal body research aspect , taking the tumor model of transplanted animals as guideline, carrying on research in tumor synergism, reducing the poison, strengthening the immunologic function and related mechanism research; on experiment outside the body aspect , using blood serum pharmacological method, with MTT, FCM, LSCM technique used , calculating the restrain expression rate of drug serum , combining with research of morphological observation, apoptosis rate check , cell cycle analysis, the expression of apoptosis control protein , the Ca²⁺ density in cells and mitochondrial membrane potential, do research on the mechanism of wenxia decoction drug serum in inducing apoptosis of colon cells.Results presenting the thought of curing and preventing the Carcinoma of the colon and rectum that the using of wenxia therapy should be emphasized; the internal body researches indicate that the wenxia decoction have positive effects such as reducing the poison, strengthening the immunologic function, meanwhile, the wenxia decoction can resist the atrophy of immune organ which was caused by the chemotherapy medicine, enhance the quantities of the TNF-a and immuno cell factors IL-6 as well; the experiments of cell culture in vitro show that the tumor inhibition effect, namely inhibition of the tumor cell proliferation, of the wenxia decoction blood serum react distinctly and optimally 24 hours later; apparent...