The Experimental Study On Treatment Of Metrorrhagia And Menorrhagia Due To Blood-Heat By Qingrezhixuening

Qingrezhixuening is considered to be the effective recipe to treat metrorrhagia and menorrhagia due to blood-heat, which is summarized from long-term clinical practice by professor baozhang Ma. To discuss hemostatic mechanism of Qingrezhixuening, we select several experimental animal models to design "models association" and use technology of in situ hybridization, immunohistochemistry, serm pharmacology, radioimmunoassay to study. Result:1.Qingrezhixuening can decrease bleeding time,coagulation time and also decrease PT, APTT of rat,which shows it has mutiple-channels coagulation function.2.Qingrezhixuening depress ear swelling made by dimethybenzene-stimulating and the ueterine inflammation made by foreign material.3.Both of solution and pharmacological serm of Qingrezhixuening can increase the force of the contraction of the ueterine smooth muscle.4.High-dose of Qingrezhixuening can increase endothelin expression in endometrium; Qingrezhixuening can promote the mRNA expression of ER and PR in ueterin tissue with in situhybridization method.5.Low-dose of Qingrezhixuening can promote the development, maturity of follicle, and facilitate ovulation and the produce of corpus luteum, increase the E₂,P in serm of immature rat. It also improve ovarian coefficient while the high-dose decrease slightly, which shows the two groups have significant difference. It prove that Qingrezhixuening has two-way regulation on the function of HPOA.