Studies On Chemical Constituents Of Verbena Officinalis L. And Fingerprint Analysis Of Flos Carthami

Two parts of researching work were included in the dissertation. The first part was the study of chemical constituents of Verbena officinalis L. (Verbenaceae), which was an important folk herb medicine. And the second was the study of fingerprint analysis of Flos Carthami (dried flower petals of Carthamus tinctorius L. (Compositae)).Verbenalin (also named cornin), hastatoside and verbascoside were the important immunoregulational compounds of Verbena officinalis L.. And verbenalin was a prominent constituent of Cornus officinalis, which was one species of Liuwei Dihuang Wan, which was a well-known Chinese traditional patent medicine and widely used. But the content of verbenalin in Cornus officinalis was lower than in Verbena officinalis L.. The methods of HPLC quantitative analysis, extraction and isolation of three main bio-active constituents above-mentioned have been established based on the chemical constituents investigating of Verbena officinalis L.. The structural modification of three were designed and prepared, too. The autoxidation of verbenalin in the extraction solution of Verbena officinalis L. was discovered and studied, its possible mechanism was described. The HPLC fingerprint analysis of Verbena officinalis L. was carried out. Furthermore, fingerprint analysis of Flos Carthami, safflower yellow pigments, safflower yellow pigments and sodium chloride injection were also studied by HPLC.1. The chemical constituents of Verbena officinalis L. have been investigated. Seven compounds have been isolated from the alcohol extract of the whole grass and they were determined as 7a,22P-dihydroxysitosterol, ursolic acid, 3