Intramyocardial Injection Of BFGF And BDNF Microspheres To Treat Ischemic Canine Myocardium

Objective To study the feasibility of direct intramyocardial injection of exogenous bFGF and BDNF microspheres to the cardiac myocytes. Methods bFGF and BDNF microspheres were constructed and they were injected into the cardiac myocytes by direct intramyocardium. Then the cardiac myocytes were detected by HE staining and immunohistochemistry. Results There was no inflammation in the injected areas. The cardiac myocytes successfully expressed bFGF and BDNF protein, respectively, which was confirmed by immunohistochemistry. Conclusion Exogenous bFGF and BDNF microspheres have good histocompatibility with cardiac myocytes. bFGF and BDNF can be expressed stably in the cardiac myocytes with the direct intramyocardial injection of exogenous bFGF and BDNF microspheres, and it may be useful for the therapy of myocardial ischemia with bFGF and BDNF...