| Background and ObjectiveThe long-term prognosis of coronary artery disease, whose mortality increases in recent years, is not optimistic, because the PCI and CABG treatments were restricted. And PCI is not more effective than drug treatment for the chronic chest pain patients.Therapeutic angiogenesis, praised as molecular bypass, can promote angiogenesis in myocardial ischemic zone and increase the myocardial perfusion in ischemic heart disease,so it is having received increasing attention as a new treatment method. The pathological neovascularization after the birth includes angiogenesis and arteriogenes, which have extremely important effect on the long-term prognosis of acute myocardial infarction ( AMI ) and heart failure ( HF ) . Angiogenesis includes the process of cells turning into capillaries and blood vessels which have blood perfusion. There are a large number of vascular growth factors and inhibitors in the process of angiogenesis. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor ( bFGF), which reduce myocardial infarct size, increase capillary density and improve myocardial perfusion in basic and clinical researchs, have the strongest function in vascular growth factors. Previous studies showed that adenosine triphosphate (ATP), carvedilol, Shexiangbaoxin pills and Danhong injection had reduced myocardial infarct size and increase the efficacy of myocardial perfusion, but their mechanism is not unknown till now. In this paper, 45 AMI rabbits model were randomly divided into 6 groups, and were administered different medicines, after 14 days, the rabbits' hearts were handled with the immunohistochemical study of VEGF,bFGF and CD34, so that we can compare and evaluate their different effect of therapeutic angiogenesis respectively, and reveal their respective therapeutic angiogenesis mechanism.Methods45 female Japan-sino hybridization white rabbits ligated anterior descening branch of coronary artery were randomly divided into 6 groups according to their different medication. Group 1: NS 2ml/kg iv qd×14, group 2: Danhong injection 2ml/kg iv qd×14, group 3 : Danhong injection 1 ml/kg iv qd×14, group 4 : adenosine 2ml/kg iv qd×14, group 5 : Shexiangbaoxinwan 50mg/kg, intragastric administration qd×14, group 6 : Carvedilol 1mg/kg, intragastric administration qd×14.14 days latter, the rabbits' hearts were removed and cut into slices, which were handled with the immunohistochemical study of VEGF,bFGF and CD34.ResultsThrer were higher expression of VEGF and bFGF in both infarction zone and border zone and the zone of non-infarction in group 2,3 and 4 than that in group 1 (p <0.05), and the same with the density of capillary(p <0.05). There was no statistical variance of VEGF and bFGF between group 5 and group 1(p> 0.05), but CD34 in group 5 was higher than that in group 1 in the border of infarction zone and in non-infarction zone(p <0.05).There was no significant difference between the VEGF in group 6 and the negative control group(p> 0.05), and the expressions of bFGF showed that there had been a significant difference between group 6 and the negative control group both in the infarction zone and in the non-infarction zone(p <0.05),and that there was no significant difference in the border of infarction zone between group 6 and group 1(p> 0.05).The results of CD34 staining showed that there had been no significant difference in the infarction zone between the group 6 and group 1 (p> 0.05), and that there had been a significant difference between group 6 and the negative control group both in the border of infarction zone and in the non-infarction zone(p <0.05).Conclusion1. Danhong injection, ATP injection and Carvedilol can not only increase the expression of VEGF, bFGF and the density of capillary, but decrease the area of the infarction zone after acute myocardial infarction. Shexiangbaoxinwan can increase the density of capillary in the border of infarction zone and the normal tissue, but it can not decrease the area of the infarction zone after acute myocardial infarction.2. In addition to the factor VEGF and bFGF, there are other factors involved in the process of therapeutic angiogenesis. |
PDF Full Text Request