Effect Of Phenylacetate On Apoptosis In Rat C6 Glioma Cell Line And Its Mechanisms

Objective: To investigate the effect of differentiation inducer-phenylacetate(PA) on apoptosis in rat C6 glioma cells and to study the mechanisms of PA.Methods: C6 cells were cultured in vitro. After induced by PA at different concentrations (2.5, 5.0 and 7.5mmol /L) , the rate of cell proliferation inhibition was measured by MTT assay at different time-points(24,48,72 and 96h). The changes of cell morphology was observed by microscope and transmission electron microscope(TEM). Apoptosis was detected by flow cytometry (FCM) with AnnexinV/ propidium iodide(PI) and TUNEL assay. The expression of Bax, Bcl-2, survivin, p53 and GFAP was determined by IC or ISH. The level of intracellular free calcium was determined by laser Confocal scanning microscopy with calcium-flourecent probes-Fluo-3/AM.Then C6 cells were transplanted into the brain of Wistar rats. PA was administered by peritoneal injection. Apoptosis was detected by TEM and TUNEL. The expression of Bax, Bcl-2, survivin, p53 and GFAP was determined by IC or ISH.Results: In vitro, the growth of C6 cells was obviously inhibited by PA, and the inhibition rate was increased with the increase of concentration and...