The Mechanisms And Effects Of MiR-21 On Proliferation And Apoptosis Of Human Brain Glioma Cells

Objective: To explore the biological function of miR-21 and the effects of its down-regulation ,a new approach was designed on proliferation and apoptosis of human brain glioma cells for diagnosis and therapy.Methods: Anti-miR-21 was synthesized chemically in vitro and transfected into U87 malignant glioma cells by liposome lipofectamine.Cell viability was assessed by cck-8 assay and apoptosis of glioma cells by flow cytometry. PDCD4 and PTEN were examined by Western blot and their genes also detected by qRT-PCR in order to find the function of miR-21.Results: Proliferation of U87 cell was inhibited and apoptosis was induced by anti-miR-21.The mRNA level of PDCD4 and PTEN did not change significantly after cell transfection compared to the control. The protein level of PDCD4 and PTEN increased after inhibition of miR-21.Conclusions: It suggested miR-21 an oncogene role which was vestified by experiment that proliferation of U87 cell inhibited and apoptosis induced by Anti-miR-21. Because the protein level of PDCD4 and PTEN increased while ever their mRNA level not changed significantly when transfected with the anti-mir-21 ,it mention us miR-21 maybe play a post-transcriptional character to several target genes of glioma . It maybe a new way and a promising molecular target for us to treat glioma in future.