Preliminary Killing Mechanism Of Zinc Chloride On Giant Cell Tumor Cells In Vitro And Study On The Related Prognostic Parameters In Giant Cell Tumor Of Bone

Giant Cell Tumor of Bone (GCT), one of the most common primary bone neoplasm, is a challenging surgical problem due to its mostly aggressive growth with tendency to recur locally, to develop in rare instances pulmonary metastases without evidence of malignant changes. Up to now there are no absolute clinical, radiographic, or histologic parameters to accurately predict the tendency of any single lesion to recur or to metastases. Zinc chloride, one of the common chemical adjuvant used clinically after extended local curettage for GCTs , has been proved to be effective in reducing the recurrent rate from 40%-60% to 14%.The recurrent reasons may be:1, the concentration of zinc chloride is not high enough to kill tumor cells locally in vivo; 2, tumor cells are resistant to the low concentration of zinc chloride. So the study on killing mechanism of zinc chloride on tumor cells and prognostic parameters in GCTs treated by curettage and cautery with zinc chloride is theoretically and practically important.The primary culture was established and the growth character in vitro, cell types, DNA flow cytometry were observed; The efficacy of different concentration of zinc chloride on tumor cells were examined; The expression of bcl-2 , bax gene products...