Human CD14 Gene Recombination, Expression In Mammalian Cells And Detection Of CD14 In Dental Tissue

CD14 is 55-kDa protein found both as a glycosylphosphatidyl inositol-linked protein on the surface of mononuclear phagocytes and as a soluble protein in the blood. CD14 on the cell membrane (mCD14) has been shown to serve as a receptor for lipopolysaccharide(LPS) of Gram-negative bacteria, but the function for soluble CD14(sCD14) has not been thoroughly understood. Gram-negative bacteria are involved with the etiology of dental infections, including endodontitis, apical periodontitis and periodontitis. It therefore suggested that CD14 may play important roles in host responses to LPS of Gram-negative bacteria in dental infections. Since the function of sCD14 is not well defined, and sCD14 may neutralize LPS, thereby inhibit inflammatory response. On one hand, it's important to elucidate the biological function of sCD14; on the other hand, it's also needed to develop ways to produce sCD14 for the aim of investigation or application. In this study we performed the following experiments: Recombine CD14 gene to create mutants suitable for soluble expression in mammalian cells; Expression of recombinant sCD14(rsCD14) in mammalian cells; purification of rsCD14 by immuno-...