Effects Of Daunorubicin And Bax Overexpression On Apoptosis Of Cultured Human Retinal Pigment Epithelial Cells And Experimental Proliferative Vitreoretinopathy In Rabbits

Purposes Proliferative vitreoretinopathy (PVR) after rhegmatogenous retinal detachment is a series of cellular events that include dedifferentiated cell formation, migration, adhesion, and proliferation, followed by membrane formation and traction along surfaces of the detached retina resulting in failure of surgical retinal reattachment. PVR is an excessively wound healing which exits imbalance between cellular proliferation and cell death. Retinal pigment epithelial cells (RPE) are major proliferative cells in PVR, the apoptosis of RPE is critical to the initiation and progress of the disease. It has been proved that bcl-2 oncogene family members and proteases can exert important modulatory effects on apoptosis of the cells. This article includes four parts: (1) Immunohistochemical detection was performed to exam the expression of bak and bcl-XL gene of fluid collection during vitrectomy for PVR grade B, and epiretinal membranes from PVR grade C or D. To analysis the correlation between the two gene expression and apoptosis. (2) To transduce the cultured human RPE with human bax gene, then induce their expression. (3) To observed influences of daunorubicin and bax overexpression on the bak and bcl-XL expression, activity of caspase-3 and apoptosis of RPE cells. (4) To observe the PVR-inducing capability of normal and bax-gene transferred RPE in rabbit eyes ,and the inhibitory effects of daunorubicin on the formation of two kinds of PVR. To study the influence of Bax overex-...