Heparanase Expression And Its Correlation With Invasion And Metastasis In Hepatocellular Carcinoma

The hepatocellular carcinoma (HCC) is one of the most common malignant tumors in our country, which is frequently associated with metastasis in the early stage and high rate of recurrence after operation or intervention treatment. To identify the principium of invasion and metastasis and to search effective approaches to prevent the recurrence and metastasis of HCC remain a challenge to modern medical science. Recently it has been reported that the degradation of the extracellular matrix(ECM), blood vessel basement membranes(BMs) and neoangiogenesis are closely involved in the invasion and metastasis of HCC. Heparanase (hpa) degrades heparan sulphate proteoglycans (HSPGs) to facilitate tumor invasion, metastasis and angiogenesis, which are the main components of the extracellular matrix and basement membrane. It is the only one we know by now that degrades the heparan sulphate chain of HSPGs, releases and activates growth factors combined with HSPGs such as bFGF,VEGF et al, and induces angiogenesis, in this way, Hpa promotes tumor invasion and metastasis. It is becoming a new target to treat cancer with the development of the study about the relationship between the Hpa and the tumor invasion and metastasis. PART I The clincopathological significance of heparanase mRNA expression in hepatocellular carcinomaObjective: The relationship was investigated between Hpa and biological characters in HCC such as invasion and metastasis by testing the expression of Hpa mRNA in HCC tissues, para-carcinoma tissues, normal liver tissue and human liver cancer cell line SMMC-7721, BEL-7402 in vitro. The aim is to clarify the mechanism of the HCC invasion and metastasis, and to provide theoretical and experimental basis on intervening the break down barriers of ECM and BMs in molecules level to inhibit tumor angiogenesis and...