Effect Of Exogenous Epidermal Growth Factor (EGF) On Recovery Of Acute Renal Tubular Injury Induced By Gentamicin

Acute renal tubular injury including acute renal tubular necrosis (ATN)is the main cause of acute renal failure. The recovery mechanism of acute renal tubular injury has not been elucidated. However, preliminary studies show that this mechanism is related to changes of exprssion and regulation of various endogenous growth factors, in which EGF is the important one. The effect of EGF in the recovery of gentamicin(GM)-induced ATN is not clear.To examing the relationship between EGF and recovery of acute renal tubular injury, we studied the effects of exogenous EGF on GM-induced ATN in rats, which include the impact of EGF on tubular cell proliferation and apoptosis, and the role of renal eicosannoids in ATN.1. Effect of exogenous EGF on GM-induced mild-moderate ATN. Female wistar rats were divided into three groups: (1). Normal rats (NL, n=7): rats were not treated; (2). GM-treated only (Group G, n=20): rats were administered with GM 200mg/kg×3 days ip;(3).GM and EGF-treatesd (Group G+E,n=19) :EGF(20ug) was injected subcutaneously after last injection of GM. [~3H] thymidine incorporation rate of renal tissue and serum creatinine concentration were measured at day 1,4,8,12 after GM administration. [~3H] thymidine incorporation rate of renal tissue in group G+E was significantly higher than that in group G at several time poits after toxic injury(at day 4...