Study On GR,HSP90 Expression In Peripheral Blood Mononuclear Cell From Seroid-sensitive,Dependant And Resistant Asthma

Objective To investigate the expression of GR,HSP90mRNA in peripheral blood mononuclear cells(PBMC) from steroid-sensitive asthma(SS),steroid-dependant asthma(SD) and steroid-resistant asthma(SR) and the role of GR and HSP90 on the pathogenesis of SR. Method With reverse transcription-polymerase chain reaction(RT-PCR) and Western blot ,the expression of GR and HSP90 were detected in PBMC from 10 normal volunteers, 10SS,5SD and 6SR as and as in vitro cultured in the absence and presence of IL-2 and IL-4. Results The expression of GR,HSP90mRNA in PBMC from SR patients(0.730± 0.171,1.122 ± 0.165 respectively) was the highest.The seconds from SS patients(0.359±0.350,0.885 ± 0.250 respectively).The lowest was from SD patients(0.017±0.008, 0.078±0.039 respectively).The expression of GR and HSP90mRNA in PBMC from normal volunteers was 0.052 ± 0.013 and 0.362±0.101 respectively.The difference of the expression of GR or HSP90 between each other of them was significant(P<0.05).The ratio of hsp90/GR in normal volunteers,SS,SD and SR patients was 7.15 ± 1.84,8.39 ± 7.95,5.51 ± 3.30 and 1.57 ± 0.18 respectively.HSP90/GR in SR was significant lower than that of the others and it suggested that the expression of HSP90mRNA was not sufficient in this patients.When PBMC from SS,SD and SR were incubated with IL-2 or IL-4 alone,no change of GR and HSP90 mRNA expression were observed. When incubated with combination of IL-2 and IL-4,a significant higher expression of GRmRNA expression was observed in all asthmatics and a significant higher expression of HSP90mRNA was observed only in SS and SD patients but not in SR patients.The level of proteins of GR and HSP90 was similar with that of their mRNA experssion.Conclusion The low of the ratio of HSP90/GR may be one of the cause of SR.IL-2 and IL-4 may play a important role in the imbalance of HSP90/GR.