Experimental Study On Induced Differentiation Of Rat Hair Follicle Bulge Cells Into Sebocytes In Intro

Previous studies discovered that Slow-Cycling High proliferate potential cells of hair follicle were localized to the prominent bulge region which is located below the opening of the sebaceous duct and serves as the attachment site of arrector pili muscle. Recently experiment demonstrated that bulge-located stem cells can not only migrate to the upper part of the follicle to generate sebaceous glands and epidermis, but also give rise to many of the compartments of the lower follicle. Because of their intimate relation, the hair, sebaceous and epidermis are collectively known as the epidermo- pilosebaceous unit or pilosebaceous unit.As an appendix organ appendage of skin, sebaceous glands play an important role of skin homeostasis. Sebum was found to transport antioxidants to the skin surface. In addition, sebaceous was found could protect the skin against oxidative stress and, especially, epidermal keratinocytes against ultraviolet B irradiation, the sebum-specific lipids to exhibit innate antimicrobial activity. Sebaceous gland development and function is regulated by an expanding array of molecules including transcription factors, hormones, cytokines and nuclear hormone receptors. PPARγplays a role in stimulating sebocyte development and lipogenesis, it regulates the late stages of sebaceous cell differentiation, and is the most effective isotype in stimulating lipid production in these cells. Although much experiment has been show that hair follicle stem cells were the source of sebaceous glands, but up to now, there have not evidence could proved the differentiation of hair follicle bulge cells into sebaceous gland cells in vitro. Isolating and cultivating stem cells, which became an obstacle to research on the characteristic of bulge cells. In this experiment, first, we isolate and culture the hair follicle stem cells of rats and observe its growth property in intro, and then induced differentiation of the bulge cells into sebocytes in intro. The main results are listed following:1. Immunocytochemical staining were used to detect expressing of K19 and K10 in rats hair follicle, hair follicle bulge obtained by micromanipulation and enzyme digestion were cultured in two different conditions, serum-free K-SFM and DMEM/F12 that added...