Studies On Genotypes Of Human Papillomaviruses In Condyloma Acuminata

Objectives To investigate molecular epidemiological characteristics of human papillomavirus (HPV) infections among patients with Condyloma Acuminata (CA), analyze the potential relationship between HPV genotypes and clinical characteristics , and compare agreement of HPV genotypes between swabs and CA tissues and finally facilitate further study on molecular epidemiology of HPV infections. Methods In the first part of the study, HPV DNA isolated from 201 CA specimens were amplified by using a PCR with PGMY09/11 primer system. Each PCR product of HPV DNA was genotyped by simultaneously hybridizing it to 37 kinds of specific HPV probes immobilized on a nylon strip (a reverse line blot assay). All DNA templates were amplified by PCR using HPV 6 and 11 type-specific primers (TSP) in order to compare PCR with the reverse line blot assay in terms of the agreement between HPV6 and 11. The correlation between HPV genotypes and clinical characteristics was priliminarily analyzed by using SSPS11.5. In the second part of the study, HPV DNA obtained from swabs and CA tissues in 70 cases with CA was amplified by PCR technique. The results were analyzed by SSPS 11.5 to determine the agreement between them regarding positive rates of HPV genotypes. In the third part of the study, the gene sequence of HPV cp6 108 found in CA specimens was studied with PCR and TA cloning techniques. Results 1.Line blot assay showed that all samples were positive for HPV DNA, covering a total of 31 genotypes and distributing as follows: type 11 (53.7%, 108/201), 6 (43.8%, 88/201), 16 (6.5%, 13/201), 52 (6.0%, 12/201), 33 and cp6108 (5.5%, 11/201 for each), 42 (5.0%, 10/201), 59 (4.0%, 8/201), 58 (3.5%, 7/201), 18, 56, 84 (3.0%, 6/201 for each), 45, 55 (2.5%, 5/201 for each), 31, 39, 51, 53, 61, 66 (2.0%, 4/201 for each), 62, 81 (1.5%, 3/201 for each), 40, 54, 67, 68, 73 (1.0%, 2/201 for each), 35, 64, 82, 83 (0.5%, 1/201 for each). Samples infected with a single HPV genotype versus multiple genotypes were respectively 60.2% (121/201) and 39.8% (80/201). Genotypes which can cause a single HPV genotype infection included type 11 (48.9%,...