| Objective: Different methods have been used to repair infracted myocardium after acute myocardial infarction(AMI). Previous studies reported transplantation of bone marrow stem cells including mononuclear cells was safe and feasible for AMI. However, little was known about the clinical role about bone marrow mesenchymal stem cells in repairing of infracted myocardium for patients with AMI. As a result, the present study aimed at resolving several issues including experimental and clinical study exploring the significant role of bone marrow stem cells in improving left ventricular function, the clinical role of cardiac electromechanical mapping(NOGA) for detection fo vital myocardium.Methods(一) Experimental study: 20 domestic pigs(male, 20-30kg of weight)were Randomly divided into two groups 24h after successful induction of acute myocardial infarction by continuous block of anterior blood flow with target diameter of balloon in left anterior desending coronary artery.30ml of bone marrow was aspirated with needle. Several centrifugation with 1000-1500rpm was underwent to separate the mononuclear cells by Ficoll-Paque liquids(1.077g/ml). Continuous culture was conducted. Mesenchymal cells suspension was prepared for injection 2h before implantation procedure. Brdu and/or PKH26 was added to the culture medium just 2 days before implantation.Protype and circles of mesenchymal cells were detected by flow-cytometer.Invasive hemodynamic parameters including left ventricular systolic pressure(LVSP), left...
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