An Analysis Of Genes Related To VSD & Clinical Application Of Echocardiography

Chapter â…  : Identification of differentially expressed genes in human ventricular septal defects using suppression subtractive hybridization[ Background ]Congenital heart defects (CHDs) account for the largest number of birth defects in humans, with an incidence of 1% in live births and 5-10% in spontaneously aborted pregnancies. As a single cardiac malformation, the ventricular septal defect (VSD) is the most frequent form of CHDs and is present in the 33% of all affected infants. To date, a few of genes, which cause CHDs, have been identified like the mutation of NKX2.5, GATA4, BMP10, etc., all of which are associated with atrial and ventricular septal defects. However, like most CHDs, VSD is thought to be multigenetic disorders, and the basic mechanism for VSD in humans is still incompletely defined. Suppression subtractive hybridization (SSH) is a PCR-based approach to analyze changes in gene expression. It is a highly effective method for generating subtracted cDNA libraries. It increases the probability of obtaining low-abundance differentially expressed cDNAs dramatically and simplifies the analysis of the...