Expression And Function Of Proteins Involved In The Effect Of Hyperthermia On Spermatogenesis In Adult Male Mice

Spermatogenesis is the process by which stem cells develop into mature spermatozoa. There are three phases: (1) Spermatocytogenesis (Mitosis), (2) Meiosis, and (3) Spermiogenesis. Many testis-specific proteins are required for mitotic proliferation of spermatogonia, spermatocytes undergoing genetic recombination and meiotic divisions, and differentiation of haploid spermatid. Isolation and identification of spermatogenesis related proteins and further functional study are very important to the elucidation of molecular mechanism involving in meiosis and spermiogenesis. It is also important to clinical diagnosis and treatment of male infertility and male molecular contraception.We characterized cellular and molecular mechanisms involved in spermatogenesis following short-term heat exposure of murine testis. For these studies we utilized a proteomic approach with two-dimensional gel electrophoresis (2-DE) analyses and mass spectrometry (MS)...