Differential Analysis Of Gene Expression Profiling And Morphology Of Human Colorectal Cancer Cells With Different Metastatic Potentiality

The tumor metastasis is an extremely complicated process involving many steps including adherence, movement of tumor cell, escaping from immunizing kill in organism, angiogenesis and other biological behaviors of tumor cells. It may be results of interaction of tumor cells and their host, and selection of subgroups of heterogeneous cells from original tumor tissue. The gene modification of tumor cells plays a dominant role during tumor metastasis. And metastasis-related genes are the key points of development of tumor metastasis. The expression of metastasis-related genes and their modification may control metastasis phenotype, and may result in morphological changes of cancer cells. Exploring mechanisms of tumor metastasis, confirming the relevant factors of tumor metastasis is very significant for judging prognosis, guiding therapy and improving the life quality of the patients.The cell line with the same genetic background and different metastatic potential is a common model to study metastasis mechanism, because the cell line with the same genetic background can resist the interfering information to the lowest level. By comparing cell form, biological characteristics, expressive gene, protein difference, etc, but such difference can be concerned as relevant with tumor metastasis, and if such difference is expressed in the cell form of biological characteristics of tumor cell, it is recognized to be related metastasis with heterogeneity.Three types of CRC cell line with the same genetic background is used in...