Relationship Between AXIN1 Gene Mutations And The Development Of Colorectal Cancers And Gliomas

Wnt and JNK signaling pathways are composed of proteins encoded by a series of oncogenes and tumor suppress genes, these proteins restrict and cooperate with each other, playing important roles in cell shape, cell movement, cell adhesion, cell proliferation, differentiation and development. Disorderliness of the signaling network is tied up with oncogenesis. Axin was initially identified as an axis inhibitor; it is also a very important tumor suppressor. As a scaffold protein, Axin has many binding domains, through which it interacts with many important components of Wnt and JNK signaling pathways, to regulate the balance of the signaling transduction together with other factors. Additionally, Axin participates in some other critical signaling pathways such as p53 signaling pathway, TGF-βsignaling pathway, and so on. The facts that Axin participates in Wnt, JNK, p53 and other important signaling pathways to regulate cell fate including cell growth, proliferation, oncogenesis etc., arose our interest to study the relationship between Axin and human oncogenesis.Cancer is a kind of grisly disease hurtful to human health. Colorectal cancer is one of the mainly lethiferous human cancers, and glioma is the most frequent tumor in the central nervous system. In this thesis, 54 samples of colorectal cancer tissues and 56 samples of glioma tissues were collected for analysis. Immunohistochemistry analysis was employed with antibody ofβ-catenin and Axin to detect if abnormity of the levels of these two proteins occurred in these samples. The genomic DNA was extracted from the 54 colorectal cancer tissues and 29 of the 56 glioma tissues. PCR amplification...