Study On Chemical Constituents Of Eucommia Ulmoides Oliv. And Two New Drugs Preparation

This paper consists of three parts.The first fraction is extraction, isolation, purification and analysis of bioactive components in Eucommia ulmoides oliv (Duzhong), as well as application of high-speed countercurrent chromatography (HSCCC).The second part is made of the extraction of gentiopicroside in Qinjiao above ground part and its film-coated tablets preparation.The last section is studing on Qingre XiaoYanling film-coated tablets production and quality control standards.In the first part,we make use of traditional silica column chromatography method to carry out a systymatic research on the chemical constituts of the bark of Eucommia ulmoides oliv.Seven compounds were obtained from the process.They are aucubin, pinoresinol diglucoside (PDG), Medioresinoldiglucoside, syringaresinoldiglucoside (SDG), olivilglucoside, pinoresinol single-glucoside,loliolide. A new, simple, and rapid technique was established for isolation and purification of PDG from Duzhong.In order to evluate the reborn barks qulity,PDG content in three times reborn barks were determined.The result indicates that the girdling bark regeneration can be made twice,the time between the first and the second is not less than five years.The research also provides an experimental basis for the girdling bark regeneration technologies and solves people's doubts about the quality of recycled bark.HSCCC is a liquid-liquid partition chromatography with a liquid stationary phase.lt has advantages of short separating process,high efficiency,simple preparation sample,convenient to operate,less solvent consumption,and eliminating irreversible adsorption.We employed the new technology in separation PDG and SDG from the crude extract of eucommia bark.The method yieded PDG and SDG over 95% purity in a single operation.To our knowledge, this is the first report and the paper about the test was accepted by SCI.Gentiopicroside is the main effective ingredients in Qinjiao underground root.Because of longer growth cycle,lower yields,we found new medicinal part in Qinjiao that was wasted every year aboveground.To our research,the content of gentiopicroside in Qinjiao overground part can meet the standard of Chinese Pharmacopoeia.So we studied the extraction technique of gentiopicroside from aboveground part of Qinjiao.The extraction was prepared into film-coated tablets.In the tablets production process,the two problems of absorbing moisture and caking softened were solved.In the last chapter,we researched the preparation and quality control standard of Qingre Xiaoyanling film-coated tablets.Thin layer chromatography (TLC) was used for identification,HPLC was employed for the contents of the main active ingredient.