Experimental Study On The Effect Of SOX9 In Hyaline Cartilage Cells Of Human Cervical Degenerative Disease

This research is was investigate the expression of Sox9 inchondrocyte of normal and degenerative human cervical zygapophyseal articulation.The reseasch firstly analysed quantitatively the changes on the Sox9 gene in thecourse of degeneration of cervical disease.To assess the influence effect of Sox9 onhyaline chondrocyte.The chondrocytes were obtained from human cervicalzygapophyseal articulation by mechanical and trypsin-collagenase digested. Phasecontrast microscope, collagen typeⅡimmunocytochemical stain were used to observethe morphological characters of chondrocytes.This method retained the mainmorphological character of chondrocyte in the period of growth.To investigate therole of Sox9 and transforming growth factorβ1(TGF-β1) in the regulation of thegene expression of matrix metalloproteinases-13(MMP-13),inhibitor of matrix metalloproteinase-1(TIMP-1) in the human hyaline chondrocyte. This study showsthat Sox9,TGF-β1 may play crucial roles at the normal chontrocytes and cervicaldegenerative diseases by regulating chontrocyte expression of MMP-13 andTIMP-1.In addition, Sox9 may have synergism effect with TGF-β1 working on theMMP-13 and TIMP-1 mRNA expression.The recombinant adenovirus vectorcontaining Sox9 gene was prepared.Sox9 and Caspase-3 play an important role in theprocess of the apoptosis of the cartilage chondrocytes. Caspase-3 is one of the mostimportant factors in the apoptosis process of the cartilage chondrocytes. It wouldbreak a new path for the gene therapy of cervical degenerative disease,which broughtforward new considerations on the pathogenesis of cervical zygapophysealarticulation disease.