The Experimental Study On The Influence Of Ping Chuan Ⅰ On The Contents Of STAT6 Protein Eotaxin Protein Of Asthma Model Rats And Their MRNA Expression Correlation Factor

Objective:Observe the influence of Chinese herbal medicine (PingChuan I ) to the asthma rat model STAT6 protein content, Eotaxin protein content and their mRNA and the contents IL-4,IL-13 and EOS numbers in the serum and BALF, inquiry into the function mechanism of PingChuan I to bronchus asthma.Method:Adopting immunohistochemical method and hybridization in situ method examination asthma rat model the expression of STAT6, Eotaxin protein and their mRNA; adopting enzyme linked immunosorbent assay(ELISA) to examine the contents IL-4,IL-13 in BALF and serum of rat, The Wright staining examine the WBC number,classification and EOS number in the BALF.Result:1.The asthma rat model group's STAT6 protein, Eotaxin protein and their mRNA's expression are obviously higher than the normal matched control, learned by covariance examination with obvious difference (P<0.01). Chinese herbal medicine low doses group, medium doses group and high doses group's the contents of STAT6 protein, Eotaxin protein is lower than model group, and their mRNA expression obviously weaker than model group, learned by covariance examination with obvious difference (P<0.01), but medium doses group, high doses group are close by dexamethasone group, no obvious difference (P>0.05).2. Model set's contents of IL-4,IL-13 in BALF and serum are obviously higher than normal matched control, learned by covariance examination with obvious difference(P<0.01). Chinese herbal medicine low doses group, medium doses group and high doses group's the contents of IL-4, IL-13 in BALF and serum are lower than that model group, but medium doses group, high doses group are close by dexamethasone group, no obvious difference (P>0.05)3. Asthma model group's #EOS and EOS% in BALF are obviously higher than normal matched control group, learned by covariance examination with obvious difference(P<0.01). Chinese herbal medicine low doses group, medium doses group and high doses group's #EOS and EOS% in BALF are obviously lower than model group's, learned by covariance examination with obvious difference(P<0.01), but medium doses group, high doses group are close by dexamethasone group, no obvious difference (P>0.05). Conclusion: The Chinese herbal medicine (PingChuan I ) may lower the contents of STAT6 protein and Eotaxin protein, and die down the expression of STAT6 mRNAs, Eotaxin mRNA in these asthma rats's airway epithelial cells, lower the contents of IL-4, IL-13 in the asthma rat's BALF and serum, lower the number of EOS in the BALF, tamper airway's inflammation, achieve the treatment purpose for the bronchus asthma.