Feasibility Study On Allogenic Transplantation Of Tissue Engineered Testeosterone-Secreting Tissue

Objective To investigate the feasibility on allogenic transplantation of tissue engineered testeosterone-secreting tissue with co-transplanted LC and SC. Furthermore, establish an isolation and culture system of human testicular LC and SC on the foundation of this technique. Methods LC and SC were got by collagenase digestion and different-speed adherence methods from the same rat or human testis, and the characteristic and purity of the isolated LC and SC were identified and analyzed. The LC and SC were seeded onto the PGA/PLA material to form cell-scaffold constructs. The cell-scaffold constructs were transplanted into the gastrocolic omentum of the castrated homogeneic or allogeneic rats. The serum testosterone was assayed at regular intervals. Results Both LC and SC can be got from the same rat or human testis by collagenase digestion and different-speed adherence methods with high purity and good function. And after isotransplantated or allotransplantated into the castrated rats, the secreting function of the constructs could last at least 3 month. Conclusion Abundant LC and SC with high pure and good function can be got by collagenase digestion and different-speed adherence methods from the same rat or human testis. It is feasible that to construst the tissue engineered testeosterone-secreting tissue with co-transplantation LC and SC in allogeneic individuals.