Studies On Bio-active Constituents Of Four Medicinal Plants

This dissertation describes the chemical and pharmacology study of Tinospora sagittata (Oliv.)Gagnep, Tinospora capillipes Gagnep, Daphne genkwa Sieb. et Zucc.and Crinum latifalium L.. From thefour medicinal plants, 97 compounds in total were obtained and indentificated, including 13 newcompounds. The bio-assay guided isolation of anti-inflammatory and analgesic activity of T. sagittata andT. capillipes were perfermed. Futhermore, High-performance liquid chromatography (HPLC) coupled withelectrospray tandem mass spectrometry (ESI-MS-MS) and diode array detection (DAD) was employed toidentify and simultaneously determine eight major ingredients in Radix Tinosporae. Genetic algorithmbased approaches were established to study the quantitative composition-activity relationship of RadixTinosporae. A method of HPLC coupled with tandem mass spectrometry (LC/MS/MS) was established forthe determination of columbin in plasma.The method was further applied to assess pharmacokinetics ofcolumbin after intravenous administration and oral administration in rats. The mechanism ofanti-inflamatation of colubin was also discussed. The bionomics and resources of Radix Tinosporae wereinvestigated. The bio-assay guided isolation of anti-inflammatory, analgesic and anti-arthritis activity of D.genkwa were perfermed. The bio-assay guided isolation of cytotoxin activity of C. latifalium was assessedby MTT method.