Effects Of BFGF Gene Mediated By Adenovirus On Biological Behavior Of HUVEC And Preliminary Study On Construction Of Hemangioma Animal Model

Objective: bFGF gene transfect HUVEC mediated by adenovirus in vitro. The biological behavior of HUVEC was changed after transfection and bFGF transfected HUVEC (bFGF-T-HUVEC) induced highly angiogenesis when injected in nude mice, aiming to construct a hemangioma animal model. Methods: The adenovirus vector harboring human bFGF cDNA was constructed by homologous recombination technique and transfect HUVEC in vitro. Detect protein and mRNA expression of bFGF and VEGF before and after transfection. Investigate proliferative ability of bFGF transfected HUVEC in vitro and its effect on angiogenesis in nude mice. Results: Construct an adenovirus vector harboring bFGF gene. bFGF gene was expressed successfully and upreguated VEGF expression in HUVEC. HUVEC with bFGF and VEGF overexpressed showed enhanced proliferative ability and trigger highly angiogenesis in nude mice. Conclusions: HUVEC with bFGF overexpressed can be obtained by gene engineer. The transfected HUVEC shows changed biological behavior and resembles hemangioma-like lesions in nude mice, providing the basis for constructing a truly hemangioma animal model.