Study On The Mechanisms And Susceptibility Of Occupational Medicamentosa-alike Dermatitis Due To Trichloroethylene

Part One Analysis of the components of Trichloroethylene used by patientsObjective To investigate the concentration of the impurity and find out whether or not the coexistent impurity presence in the Trichloroethylene which cause to the Occupational medicamentosa-alike dermatitis due to trichloroethylene in the factory, and to give the basis data for the role of trichloroethylene in the mechanisms of the Occupational medicamentosa-alike dermatitis due to trichloroethylene.Methods Using HP 6890/5973 Gas chromatogram-mass spectrum instrument, 0.4ml of trichloroethylene of 9 samples was taken out to detect, which is used in the factories by the patients of Occupational medicamentosa-alike dermatitis due to trichloroethylene. The graph of mass spectrum of every peak was compared with the graph of mass spectrum bank after separated by the gas chromatogram. The substance was confirmed when the identical rate is over 90%. All the components and all the samples were analyzed. We observed whether or not the coexistent impurity presenting in the samples. And if the coexistent impurity is exist, the possibility of hypersusceptibility was estimated.Results Some of the components are the same in the 9 samples come from 9 different working place. The coexistent impurity are trichloroethylene, 1-1-2-trichloroethane and tetrachloroethylene in all the 9 samples. In addition, trichloromethane, dimethylbenzene and carbon tetrachloride were detected in the 2/3 samples.Conclusion Except of trichloroethylene, all the samples have coexistent impurity: 1-1-2-trichloroethane and tetrachloroethylene. But the hypersusceptibility of the two matters has not been reported. It is suggested that the sensitinogen of the Occupational medicamentosa-alike dermatitis due to trichloroethylene may be trichloroethylene.Part TwoThe main changes of immune function on the patients of Occupational medicamentosa-alike dermatitis due to trichloroethyleneObjective To investigate changes of some immune function indexes on the patients of the Occupational medicamentosa-alike dermatitis due to trichloroethylene, understand the changes of the body and to provide a clue of the mechanisms of the disease.Methods Blood of the patients was used in vitro. The differences of proliferation of the lymphocyte induced by trichloroethylene were compared with control group. The changes of the move region of the leukocytes and the ratio of CD4+/CD8+ of the patients were also compared with the control group.Results Trichloroethylene (40mg/L and 80mg/L) could significantly increase the proliferation of lymphocytes of the patients of the Occupational medicamentosa-alike dermatitis due to trichloroethylene, and no differences of the ratio of CD4+/CD8+ has been found in the patients compared to the control group. Trichloroethylene had no significantly differences to the inhibition exponent of the leukocyte moving compared to the control group.Conclusion It is suggested that the trichloroethylene may be the antigen or hapten of the immune reaction in the Occupational medicamentosa-alike dermatitis due to trichloroethylene. Part ThreeStudy on the changes of immune functions in mice induced by trichloroethyleneObjective The experiments of plantar swelling test and lymphocyte proliferation in NIH mice were used to investigate the changes of the immune functions induced by trichloroethylene, and give evidence for the hypersensetivity of trichloroethylene.Methods The organ coefficients changes of thymus, spleen, and liver, the experiments of plantar swelling test and lymphopoiesis test were carried out by hyodermic to mice.Results The organ coefficients of thymus and spleen had no obviously changes in the mice induced by trichloroethylene. The coefficient of plantar swelling test in mice were obviously higher in 24h and 48h induced by trichloroethylene compared to control group. Trichloroethylene (80mg/L) could inhibit the proliferation of spleen lymphocytes compared to the control group. but could promote the proliferation of spleen lymphocytes of the mice.Conclusion TCE may be related with the induction of the delayed type hypersensitivity in NIH mice.Part FourStudy on the susceptibility related to immune genes in the Occupational medicamentosa-alike dermatitis due to trichloroethyleneObjective To reveal the susceptibility genes of the Occupational medicamentosa-alike dermatitis due to trichloroethylene according to the frequency of allele genotypes of HLA-DM,HLA-DQA and Lag-3 in the patients and the controls, to provide evidences for the preventive and treatment of the disease.Methods We extracted the DNA of lymphocytes of the Occupational medicamentosa-alike dermatitis due to trichloroethylene patients and controls, amplified the HLA-DM,HLA-DQA and Lag-3 by using touchdown PCR, Then through restriction fragment length polymorphism (RFLP) and sequence base typing, confirmed the alleles and genotypes. Compared the frequency of alleles and genotypes of HLA-DM, HLA-DQA and Lag-3 of the two groups.Results Our study shows that in the Occupational medicamentosa-alike dermatitis due to trichloroethylene patients a significant increase in the HLA-DMA*0101 allele frequency. The allele Frequency of HLA-DMA*0103 increased in patients group than controls. The ratio of HLA-DMA*0102-*0102 in patients is significantly higher than in controls. The ratio of heterozygote HLA-DMB *0101-*0102 in patients group is lower than controls group. And no differences were found in other alleles and genetypes of HLA-DMA and HLA-DMB.The frequency of *0201 in patients was higher than in control groups. Though the frequencies of *05- was lower than controls, but the correct P was higher than 0.05.No differences had been found in exon 1-6 and exon8 of patients and control group. Though there were polymorphism in exon 7 , but the frequencies of it had no differences in two groups.Conclusion The polymorphisms of HLA-DM, HLA-DQA may be related to the susceptibility of the Occupational medicamentosa-alike dermatitis patients, and polymorphisms of Lag-3 may be not.Part FiveResearches on changes of gene expression of the Occupational medicamentosa-alike dermatitis due to trichloroethylene patientsObjective To research the differences of gene expression of the Occupational medicamentosa-alike dermatitis due to trichloroethylene patients between prêt-reatment and convalescence, and confirmed by using real time PCR in other patients. By observed the changes of pre- and post-treatments , understand the mechanism of the disease and provided the data.Methods The total RNA were extracted from the samples of 12 patients in pre- and post-treatment. Micro array had been used to test the gene expression changes in 2 patients. Compared results of the two, the mutual changes had been found. By using real time PCR, the highest 10 different expressed genes had been confirmed in other 10 patients.Results In patient No.506, there were 317 genes differently expressed between pre-treatment and post-treatment. 262 genes of them were up-regulated and 55 of them were down-regulated. In patient No.507, there were 591 genes differently expressed. And the number of up- and down-regulated genes were 467 and 124 separately. In the two patients, 147genes were the same of up-regulated, and 9 genes were the same of down-regulated. The results showed that the functions of the differently expressed genes mabe be involved in cell surface antigen, cytokine and other genes related to immune system.Conclusion: The results showed that there were some similar changes of genes in different patients, and maybe these genes induced similar pathologic processes.