The Experimental Study On The CTGF MRNA And The Kidney Organization Cell Perishes Weakly Influence Of Shenbaining To Diabetes Nephrosis Rats Model

Objective:We observed systematically curative effect of preventing and treating Diabetic nephropathy(DN) rats by Shenbaining through clinical and experiment researches.We inquired into mechanism of traditional Chinese medicine(TCM) on DN and mechanism of Shenbaining on Preventing and treating DN.Methods:In the study,we use the left kidney of the STZ-induced DN rat.The TCM group will dissolve shenbaining in saline, prepared into suspension and it was given 2.97g/kg,1 time/day, orally,2ml/time. The positive control group lotensin was prepared into a suspension,and also was given 0.9mg/kg,2ml/time, 1time/day, orally.Control group and the sham group to the same volume of saline solution 2m,ltime/day.Then we observed the condition of their urine protein excretion,urine NAG,ACR,rat kidney weight and body weight changes.Using immunohisto chemistry,RT-PCR and color image analysis system to qualitative and quantitative detection of kidney TNF-α,Bax,Bcl-2,ColⅣ,LN, FN and expression of CTGF mRNA.Detect cell apoptosis with the original end-labeling method.Results:12 weeks later,it can be found in the control group that glomerular atrophy ball, balloon chamber slightly increased the glomerular afferent artery with slight endothelial cell hyperplasia and fibrosis,basement membrane thickening slightly,distal convoluted tubule expansion,renal interstitial congestion,edema,blood cell infiltration and mild interstitial fibrosis.Compared with the positive control group,the TCM group's body weight increase was significantly better,the kidney weight index were decreased,but the difference was not significant. At the 12th week,the TCM group urine albumin,NAG,ACR was significant different from the control group and the sham group;TNF condition of the TCM group were significantly lower with;Glomerular Bax, Bcl-2 expression of almost all groups were negative,tubular in Bax, Bcl-2 expression in sham group was significantly lower than the other three groups,the TCM group and the control group had significantly lower apoptosis,Bax expression is low as the expression of Bcl-2 higher,the TCM group and the positive control group showed no significant difference. The control group,the TCM group and the positive control group′s renal CTGF mRNA were higher than the sham operation group, there were significant differences.The TCM group,positive control group and blank control group expression is low,There was a significant difference between the TCM group and the positive control group.Conclusion:Shenbaining can increase the DN rat'weight, improve the kidney weight/body weight ratio,significantly reduced renal CTGF mRNA content, TNF-αlevels,improve urinary albumin protein, NAG, ACR,inhibit renal apoptosis,decrease the expression of Bax,Bcl-2,increased the expression of glomerular and tubular structure to protect and improve renal function. NAG,ACR and microalbuminuria are more sensitive than the response to renal injury in control of renal hypertrophy.