| Objective: The issue surrounding the NF-κB / IκB-αpathway and the theory of astrocytes observed Buyang Huanwu Decoction capsule on Alzheimer's disease (AD) rat model nerve cell injury and further explore its mechanism.Methods: Targeted by rats with bilateral hippocampal injection of Aβ1-40 to copy AD rat model , observed Buyang Huanwu Decoction capsule on behavior infection of AD rat model, observed morphological changes by HE staining and transmission electron microscopy , determined serum Aβ1-40 andβ-APP content changes by ELISA, determined NF-κBp65, IκB-α, GFAP and S100βprotein expression by immunohistochemistry, observed NF-κBp65mRNA, IκB-αmRNA and BACE1mRNA gene expression by Real time PCR , discussed Buyang Huanwu Decoction capsule protect effect nerve cells of AD from the Behavior, cellular and molecular level.Result: 1. Rat behavior experiment Buyang Huanwu Decoction capsule can significantly improve the abnormal animal behavior models, improve learning and memory. 2. Buyang Huanwu Decoction capsule can improve the brain of experimental animals by pathological factors, the abnormal morphology. 3. Buyang Huanwu Decoction capsules can significantly reduce AD rat serum Aβ1-40 andβ-APP levels. 4. Buyang Huanwu Decoction capsule can inhibit the AD hippocampus of rat brain GFAP, S100βand NF-κBp65 protein expression, the promotion of IκB-αprotein. 5. Buyang Huanwu Decoction capsule can promote AD rat hippocampus IκB-αmRNA gene expression, decreased NF-κBp65mRNA and BACE1mRNA genes.Conclusion: Buyang Huanwu Decoction capsule has a protective effect on rat neurons in AD, the mechanism may be the AD rats by reducing nuclear transcription factor NF-κBp65, thereby inhibiting the proliferation of astrocytes and reduced glial cell associated protein GFAP, S100βexpression, and inhibition of BACE1 transcription and translation of the APP activity and ultimately reduce the formation of Aβ1-40, thus preventing the occurrence and treatment of dementia. |
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