| Objective: Through investigate the effection of She Gan Ma Huang Tang (SGMHT) on the expressions of PPAR-γ,DC and TGF-β1 in chronic asthma mice,to explore the mechanisms of SGMHT treating on asthma,and to provide experimental basis for its clinical application.Methods: 60 clean female BALB/c mice were divided averagely into six groups according to the random number table,as follows:A(control group),B( asthma group),C(dexamethasone group),D(low dose SGMHT group),E(middle dose SGMHT group),F(high dose SGMHT group).GroupB,C,D,E,F were esta- blished model of chronic asthma.Group D,E,F were gastric irrigated with different doses of SGMHT;group C was intraperitoneal injection of dexamet- hasone.After drawn the materials in each group,detected TGF-β1 content in BALF by ABC-ELISA ;assessed the bronchial inflammatory cell infiltration and goblet cell ratio by semi-quantitative;measured the thickness of vascular wall, thickness of airway smooth muscle,collagen content of around airway vessels and perivascular by pathological image;observed DC morphology by electron microscopy;measured the numbers of DC, TGF-β1 and PPAR-γin the lung tissue by immunohistochemical staining;measured the expression of PPAR-γmRNA in lung tissue by RT-PCR;measured the expression of PPAR-γin nuclear protein by Western blot;measured the expression of TGF-β1mRNA in lung tissue by real-time PCR.Results:1.Compared with group A,group B's inflammatory cell infiltrati- on,goblet cell scores and theleukocytes,EOS,lymphocytes in BALF increased, there were significant differences(P<0.05);compared with group B,these indi- cators decreased in group D,E,F,there were significant differences(P<0.05); compared with group C,these indicators were similar in groupE and F(P>0.05 );group E and F were similar(P>0.05).2.Compared with group A,asthma attacks,thickness of vascular wall and airway smooth muscler,collagen content of around airway perivascular and vessels increased in group B,there were significant difference(P<0.05);comp- ared with group B,these indicators decreased in group E and F, there were significant differences(P<0.05);compared with group C,these indicators were similar in group E and F(P>0.05);group E and F were similare(P>0.05).3.Compared with group A,group B's the number of DC increased,there was significant difference(P<0.05);in group D,E,F,the number of DC is lower than group B(P<0.05);compared with groupC,groupE and F were similar(P> 0.05);groupE and F was similar(P>0.05).Observe DC morphology in lung tissue by electron microscopy, groupA and B were typical show, group C,D,E, F were untypical show.4.Compared with group A,the number of PPAR-γ,the expression of PPA- R-γmRNA and PPAR-γin the nuclear protein increased in group B,there were significant difference(P<0.05);compared with groupB,groupC's indicators are decreased,group E andF's are increased,there were significant difference(P< 0.05);group E and F were similar(P>0.05).5.Compared with group A,the content of TGF-β1 in the BALF,the number of TGF-β1 and the expression of TGF-β1mRNA in lung increased in groupB(P <0.05);in group D,E and F,these indicators are lower than group B(P<0.05); compared with group C,group E and F's indicators were similar(P>0.05); groupE and F were similar,there were no significant difference(P>0.05).Conclusion: 1.SGMHT could reduce the number of DC in lung tissue of asthmatic mice.2.SGMHT could reduce the content of TGF-β1 in BALF,the expressions of TGF-β1 and TGF-β1mRNA in lung tissue of asthmatic mice.3. SGMHT could increase PPAR-γnumber of lung,enhance the expression of PPAR-γmRNA and PPAR-γof nuclear protein in lung tissue of asthmatic mice. 4.This experiment prompted SGMHT could influence the expressions of DC, TGF-β1 and PPAR-γ,inhibit airway inflammation and remodelling, so as to achieve the purpose of treatment of asthma. |
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