| Objective:We confirm significative metabolic product related allergic bronchial asthma by metabolomics based on New Zealand rabbits asthma model.The objective is consummate the research method of the asthma's mechanism that xianweibaojin medication treating and exploring the new pattern of the reaserch in asthma model's metabolites group variation.Method:After 7 days with New Zealand Rabbits by adaptive breeding we give model group and treatment group rabbits 10% Ovalbumin(1.2ml/kg) by Celiac injecting. Normal group rabbits are given equivalent doses of physiological saline.Every rabbit is injected one time.After 14 days we atomize model group and treatment group rabbits with 1% OVA solution by physiological saline. Normal group rabbits is atomized with physiological saline.We can't stop atomizing until rabbits express abdominal muscle twitching, fidgety, nose and lips cyanosis, urinary and fecal incontinence. The atomization lasting 7 days and 1 time everyday.The fuorth day after atomizing we use irrigation stomach method to treat asthma rabbits.We determine 3 days to irrigate stomach because of the characteristics of the moderated and lasting efficacy of Chinese medicine compound. According to the conversion formula with people to animal we get the powder quantity of irrigation stomach is 0.35g.The specific operation:1.We give treatment group rabbits 15ml solution that containing 0.35g powder and 14.65ml warm water.2. Fixing the treatment group rabbits in the Rabbit box,then injecting the 15ml solution through gastric tube. (Normal group and model group rabbits are given the same dose physiological saline by the same method.)After irrigating stomach we gather Venous blood, bronchial alveolar lavage fluid and urine to detect eosinophil and metabolic product.We choose SASV8 software to analyse eosinophil count by wilcoxon and to analyse metabolic product by Oneway ANOVA.The measurement data are expressed by x±s.Result:1.The eosinophil count of blood serum and bronchial alveolar lavage fluid in model group are higher than normal group (P<0.05).2. The metabolic product of blood serum sample's statistics analysis resaults: isocitric acid:Compared with the model group, the isocitric acid expression was significantly higher than normal group (P<0.05).fructose:Compared with the model group, the fructose expression was significantly lower than normal group (P<0.05); Compared with the model group, the fructose expression was significantly lower than treatment group (P<0.05)glucose sour:Compared with the model group, the glucose sour expression was significantly lower than normal group (P<0.05); Compared with the model group, the glucose sour expression was significantly lower than treatment group (P<0.05)6-deoxidizing pyranoid mannose:Compared with the model group, the 6-deoxidizing pyranoid mannose expression was significantly lower than normal group (P<0.05).stearic acid:Compared with the treatment group, the stearic acid expression was significantly lower than model group (P<0.05); Compared with the normal group, the stearic acid expression was no difference to model group (P>0.05).half of lactose hyaluronic acid:Compared with the model group, the half of lactose hyaluronic acid expression was significantly higher than normal group (P<0.05); Compared with the treatment group, the half of lactose hyaluronic acid expression was no difference to model group (P>0.05).3. The metabolic product of bronchial alveolar lavage fluid sample's statistics analysis resaults:glycine:Compared with the model group, the glycine expression was significantly higher than normal group (P<0.05); Compared with the model group, the glycine expression was significantly higher than treatment group (P<0.05)L-aspartic acid:Compared with the model group, the L-aspartic acid expression was significantly higher than normal group (P<0.05); Compared with the model group, the L-aspartic acid expression was significantly higher than treatment group (P<0.05).tetradecaoic acid:Compared with the model group, the tetradecaoic acid expression was significantly higher than normal group (P<0.05); Compared with the model group, the tetradecaoic acid expression was significantly higher than treatment group (P<0.05)half of lactose::Compared with the model group, the half of lactose expression was significantly higher than normal group (P<0.05); Compared with the model group, the half of lactose expression was significantly higher than treatment group (P<0.05)4. The metabolic product of urine sample's statistics analysis resaults:serine:Compared with the normal group, the serine expression was no difference to model group (P>0.05). hydroxyl ethyl benzene:Compared with the treatment group, the hydroxyl ethyl benzene expression was significantly lower than model group(P<0.05); Compared with the model group, the hydroxyl ethyl benzene expression was no difference to normal group (P>0.05).xylitol:Compared with the treatment group, the xylitol expression was significantly lower than model group (P<0.05); Compared with the model group, the xylitol expression was no difference to normal group (P>0.05).5-bis hydroxy benzoic acid:Compared with the treatment group, the5-bis hydroxy benzoic acid expression was significantly lower than model group (P<0.05); Compared with the model group, the5-bis hydroxy benzoic acid expression was no difference to normal group (P>0.05).stearic acid:Compared with the treatment group, the stearic acid expression was significantly lower than model group (P<0.05); Compared with the normal group, the stearic acid expression was significantly higher than model group (P<0.05).Conclusion:1.We Simulate New Zealand Rabbits allergic allergic bronchial asthma model with injecting ovalbumin in celiac successfully.2.The content of glycine, L-aspartic acid, tetradecaoic acid and half of lactose of bronchial alveolar lavage fluid in the model group higher than normal group.We confirm these four metabolic products have the close correlation to allergic bronchial asthma.3.These four different metabolic products' content of bronchial alveolar lavage fluid in the treatment group below the model group obviously.The treatment group's content is close to normal group. The description above explain that xianweibaojin medication can repair the Metabolic network and recovery allergic bronchial asthma by specific change.4.The content of isocitric acid, fructose,6-deoxidizing pyranoid mannose.half of lactose hyaluronic acid of blood serum are changed when the rabbits catch allergic bronchial asthma. We confirm these four metabolic products have the close correlation to allergic bronchial asthma.5.The content of isocitric acid and stearic acid of blood serum in the treatment group are lower than the model group. The content of fructose, glucose sour and 6-deoxidizing pyranoid mannose of blood serum in the treatment group are higher than the model group. We confirm these five metabolic products have the close correlation to xianweibaojin compound medicine.6. The content of stearic acid of urine in the model group are lower than the normal group. We confirm stearic acid have the close correlation to allergic bronchial asthma.7. The content of serine, hydroxyl ethyl benzene, xylitol and 5-bis hydroxy benzoic acid of urine in the treatment group are lower than the model group. We confirm these five metabolic products have the close correlation to xianweibaojin compound medicine. |
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