Study On Efficacy Substances And The Mechanism Of Jinxuan Zhike Xunxi San

Jinxuan Zhike Xunxi San is New Traditional Chinese Medicineâ…¥, which is exclusive species of Mayinglong Pharmaceutical Group Co., LTD and has sold more than a year. In clinical it could be used to cure kinds of hemorrhoid such as external hemorrhoids, incarcerated hemorrhoid, combined hemorrhoid, et al. This preparation is composed of Natrii Sulfas Exsiccatus,Herba Portulacae,Flos Lonicerae Japonicae,Calcine Alumen,Herba Schizonepetae that can clear away heat and toxic material, expel pathogenic wind, swelling, ease pain. But Jinxuan Zhike Xunxi San uses the tradition manufacturing technique, which has many problems, such as the quality control standard is unperfect, the material base of pharmacal efficacy is not explicit and so on. In addition, the oversized dosage leads to high production costs.The paper applies the research techniques of the multi-disciplinary overlapping, and uses the modern science and technology, has conducted the research to the efficacy substance of anti inflammatory and antipruritic of Jinxuan Zhike Xunxi San, has discussed the mechanism of its anti-inflammatory, has interpreted the material basis for efficacy of Jinxuan Zhike Xunxi San that what the substances play a role are and how to play a role, which are two critical issues that need to be addressed.Under the guidance of Chinese medicine theory, Three taste plant medicines were regarded as a whole in this traditional Chinese medicine preparation, we used common key technologies in modern Chinese medicine theory, Which are the pocket polymeric adsorbent purification technology and the supercritical CO2 extract technology, its extraction was separated into different chemical substance spot which are the material of supercritical CO2 extract, pocket polymeric adsorbent ethyl alcohol elution spot,and pocket polymeric adsorbent water elution spot.By pharmacological study of the Wistar rat foot swelling experiments, xylene-induced ear edema in mouse experiments and itching threshold caused by histamine phosphate in the Wistar rat, the results show that the efficacy of the reconstituted effective substance compared with the original preparation is similar, so it provide a reference for reforming the traditional production process of Jinxuan Zhike Xunxi San.Using tumescent ear model in mice without adrenal induced by xylene, the migration of leukocytes in air chamber because of CMC and the peritonitis inflammation model induced by carrageenan, We studied on the anti-inflammatory mechanism of efficacy substances in Jinxuan Zhike Xunxi San. The results indicats that efficacy substances in Jinxuan Zhike Xunxi San play an anti-inflammatory effects through depending on pituitary-adrenal axis, affecting on the release of PGE2, inhibiting the leukocyte's number of sites of inflammation, reducing inflammatory factors, further interpreting the efficacy material basis of the traditional Chinese medicine.Anlysising by gas chromatography-mass spectrometry (GC-MS),we isolated and identified chemical compositions of supercritical C02 extractive from Jinxuan Zhike Xunxi San After extracting three herbals in Jinxuan Zhike Xunxi San,we determined flavonoids, saponins and total organic acids purified through macroporous resin in effective fraction by UV-visible spectrophotometry and potential back titration method, and established a reliable method for the determination. Above methods in the study on efficacy substances provide the basis for the material basis in the Chinese herbal compound, and improve the quality standards of Jinxuan Zhike Xunxi San. It provides the basis for effectivly controlling the quality of the traditional Chinese medicine products.The research findings of this paper interpret Jinxuan Zhike Xunxi San'material basis of anti-inflammatory effecacy and mechanism. They provide a scientific basis for the Chinese herbal compound'clinical effact, and a academic basis for clinical promotion to improve the market competitiveness of Jinxuan Zhike Xunxi San and create a better economic and social benefits. At the meantime, it lays a certain foundation for the secondary development into a variety of innovative raditional Chinese medicines.