| The traditional Chinese medicine Jingangteng, also known as Smilax china L., which belongs to the Lillacaee family. Jingangteng is a perennial deciduous climbing shrub whose root commonly be used as Chinese medicine. Jingangteng was recorded in the Chinese pharmacopoeia (2010 version), and has various pharmacological effects including relieve siekness wet, disintoxication and so on. The traditional Chinese medicine preparation Jingangteng capsule, which are made from the main ingredient of Rhizoma Smilacis Chinae, has good therapeutice effect against variouse gynecological inflammation. In this paper, Jingangteng effective anti-inflammatory substances (including flavonoids, saponins and tannin) was abtained based on our laboratory previous research, a systematic pharmacological research was carried on to interpreting its effective material basis and how the effective substances act.To consult domestic and international documents of Smilax china L., including botany, phytochemisty, pharmacological, and clinical literature.According to the literature, Jingangteng flavonoids, saponins and tannins have anti-inflammatory anti-bacterial effect. Jingangteng effective substances (including 33%flavonoids,13%saponins and 29%tannins) was purified by solvent and macroporous resin. Jingangteng flavonoids were adsorption by polyamide chromatography with the tannin sedimented with gelatin and saponins eluted, then Jingangteng flavonoids were gotten which containing 74%of the main effective substance. This work provides a material basis for discussing the mechanism of anti-inflammation of Jingangteng flavonoids.Systematic pharmacological research was carried on the anti-inflammatory and Analgesic Effects of Jingangteng effective substances (JES). The models of chronic pelvic inflammation disease (CPID) were made by injection of brei admixed with phenol and tragacanth into the uterus of rats, and the inhibitory effect on CPID was observed; the severity of oedema in inflamed animal was observed to study the anti-inflammatory effects of JES. The analgesic effect of the drug was studied with pain model of mice induced by acetic acid. The results show that JES could obviously inhibit the inflammation of uterus of rats with CPID; the number of stretching induced by acetic acid was reduced and the pain threshold of mice was increased by JES; JES also had anti-inflammatory activity against xylene-induced mouse ear swelling and albumen-induced rat paw edema. It could be concluded that JES has significant analgesic and anti-inflammatory activities, which are even superior than that of the Jingangteng capsule.Inhibition of bacterium activation of Jingangteng effective substance was studied in vitro. The results showed that Jingangteng effective substance had a strong antimicrobial effect against Staphylococcus aureus, Jingangteng effective substance can also inhibit neisseria gonorrhoeae and Jingangteng capsule did not.Jingangteng effective substance was intragastrically administered to mice with maximum concentration prepared (0.108g/ml) and the maximum stomach capacity (1.0ml/20g body weight), twice per day and the total dose of 10.8g/Kg per day (equivalent to 216 times the adult daily dose). Then the immediate toxicity after administration and survival within seven days was observed. The results showed that there was no acute toxicity reactions observed, all the mice survived and gain weight. Acute toxicity tests showed that Jingangteng effective substance was safe and nontoxic between the observation period.To study the anti-inflammation mechanism of Jingangteng effective substance, the models of chronic pelvic inflammation disease (CPID) rats were made by injection of brei admixed with Phenol and tragacanth into the uterus of rats, and the PGE2,5-HT and HA contents in the uterus of CPID rats were measured by enzyme-linked immunosorbent assay (ELISA) and fluorescence spectrophotometry. It was shown that Jingangteng effective substance can significantly decrease the level of PGE2,5-HT and HA in the uterus of CPID rats. we may draw the conclusion that decreasing the level of inflammatory media may be one of the anti-inflammation mechanisms of Jingangteng effective substance.An anti-inflammatory model in vitro was established based on the TPH-1 cells with which the selectively inhibition of COX-1 and COX-2 be studied. Then Jingangteng effective substance, Jingangteng flavonoids, astilbin and astragaloside were studied based on this anti-inflammatory model in vitro. The results show that the four substances all had certain selectivity on the inhibition of COX-2, IC50 (COX-1)/IC50 (COX-2) was greater than 1. But the inhibitory effect of Jingangteng effective substance and Jingangteng flavonoids was significantly higher than the other two monomers, which indicated the multi-component synergy of Traditional Chinese Medicine.The effect of Jingangteng effective substances on the cytokines IL-1β, IL-6 and TNF-a induced by LPS on THP-1 cells was studied. The results showed that the materials can effectively decrease the formation and release of IL-1p, IL-6 and TNF-a, and can reduced the mRNA expression of IL-1β, IL-6 and TNF-a, and that the inhibitory effect of Jingangteng effective substance and Jingangteng flavonoids was more significant. It can be speculated that Jingangteng effective substances exert their anti-inflammatory action by reducing the formation and release of the cytokines IL-1β, IL-6 and TNF-α. |
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