The Mechanism Of Jiangtangsanhuang Tablet Influence The Apoptosis Of Rat β Cell

The first part:literature researchCurrently, the diabetes has already become the third severity endanger in the chronic disease which to harm human's health, its incidence will rises up year by year, it's said the world to will have near500,000,000people to suffer from diabetes in2030. The findings of2008show that in our country there will be a hyperglycemia state in each four adults, so far, the mankind haven't yet find out a effecient method to cure diabetes, this also means the diabetic needs to be cure all the life. The treatment of the diabetes means to solve the insulin resistance and insulin to lack, is divided into the insulin treatment and the non-insulin treatment. The insulin treatment mainly is applicable to the patient of T1DM and parts of the patient to lose efficacy with orally drugs, or the patient who has contraindication in T2DM. orally drug include insulin promote secretion drug and non-insulin promote secretion drug,such as sulphanylureas, repaglinide, thiazolidinediones, biguanides, α-glucosidase inhibitor. glucagon-like peptide-1and dipeptidyl peptidase-IV inhibitor is used recent years. The Chinese medicine calls Diabetes as Xiaoke, and also have the name of Xiaodan, Feixiao, Zhongxiao, Gexiao and etc. The medical scientist has study it in many aspect, and make a great deal research results. Jiangtangsanhuang Tablet is invented by professor Xiong manqi, who abstraction it form Shang hanlun. From80's years, our people study it in many aspect, and make a great deal research results, and the drug is widespreadly used in clinic, obtained good curative effect,The second part:experimental researchObjectiveSudy the mchanism Of jiangtangsanhuang Tablet influence the apoptosis of rat β cell,to investigate the curative mchanism Of Jiangtangsanhuang Tablet, MethodsTest part:Take3week-ages SPF class Wistar rat, Male,5. Break the neck to death, take the pancreas get out the pancreas island cell culture. Take4adult domestic rabbits, divided into a blank group, Jiangtangsanhuang Tablet group, to feed common animal feeds, after1week to infuse stomach. After7days infusing stomach, anaesthetized with B ether, takes blood form the abdominal aorta, Take the serum to back up for further use.1,MTT test:use culture solution to feed the cells3days, divided into a culture solution group, high glucose group, high glucose and Jiangtangsanhuang Tablet group.2, ELISA test: use culture solution to feed the cells3days, divided into culture solution group and high glucose solution group, and culture solution group divided into culture solution group, culture solution and Jiangtangsanhuang Tablet group, culture solution and non-drug serum group; high glucose solution group divided into high glucose solution group, high glucose solution and Jiangtangsanhuang Tablet group, high glucose solution and non-drug serum group. After72hours to test. Use the metherd of Annexin V-FITC and PI to MTT test, use the metherd of ELISA to test IKBKB, NFKBIA, NF-kB, BAX. The dates is showed as mean±standard deviation (X±S), use analysis of variance to analyses measurement data, use SPSS13.0tools, when p<0.05statistical significance is accepted. Clinical part:48patients from in-patient department of the First Affiliated Hospital of the University of TCM of Guangzhou, divided into treating group and control group, after4weeks, take the serum, test the level of IKBKB, NFKBIA, NF-kB, BAX. The dates is showed as mean±tandard deviation(X±S), use analysis of variance to analyses measurement data, use SPSS13.0tools, when p<0.05statistical significance is accepted.RusultsTest part:MTT test reveals that the rate of apoptosis of culture solution is5.05±0.2%, the rate of apoptosis of high glucose solution is15.33±1.30%, the rate of apoptosis of high glucose solution and Jiangtangsanhuang Tablet solution is10.43±0.57%, P<0.05, and has obviously difference. In the groups with Jiangtangsanhuang Tablet, the level of IKBKB, NF-kB and BAX is remarkably lower than other groups, and NFKBIA is higher. P<0.05, and has obviously difference. Clinical part:In treating group the level of TKBKB, NF-kB and BAX is remarkably lower than other groups, and NFKBIA is higher. P<0.05, and has obviously difference. ConclusionJiangtangsanhuang Tablet has obviously effect to restrain the apoptosis of islet cell,perhaps the function is accomplished by adjust the expression of Bcl-2protiens. Jiangtangsanhuang Tablet also has the function of decrease the level of NF-kB, indicate the effect to restrain the apoptosis of islet cell.