The Impact Of Jiangtangsanhuang Tablet On The Cardiovascular Cell Induced By Glucose And Clinical Research

Diabetes (diabetes mellitus, DM) is a group which causes together by the heredity and the environmental factor take the sugar metabolic disorder as the main performance clinical syndrome. The world diabetes prevalence rate increases rapidly, the developing country is especially obvious, already became clinical on the main endocrine metabolic disease. According to the international diabetes alliance (IDF) estimated that up to2025, our country diabetic population will achieve59,300,000t. Diabetes heart disease (Diabetic Cardiopathy, DC), refers to the heart pathological change which diabetes merge or the partner send, this sickness name first proposed in1979by Ledet, obtains the world colleague expert to approve very quickly. This sickness including coronal atherosclerosis heart disease, diabetes myocardiopathy, capillaries pathological change and nervus autonomici function disorder result arrythmia and so on[3]. These kind of sickness coexist abnormal, the cause and effect relationship, promotes, to form the vicious circle mutually. Aorta's gruel type hardening (carotid artery, aorta, femoral artery) increased myocardial infarction, the stroke and the amputation danger. Diabetes myocardiopathy (Diabetic cardiomyopathy) first by Rubler and so on when the dissection contracts the congested heart failure the diabetic corpse proposes, its pathology performance mainly for heart result and so on capillaries pathological change, cardiac muscle metabolic disorder and cardiac muscle fibrosis cardiac muscle widespread textural anomalies, causes the left ventricle to be plump finally, relaxing period and (or) systole function barrier one kind of disease condition. Regarding myocardial cell's exploration, has become diabetes myocardiopathy's research hot spot at present, the use raise myocardial cell, explores the non-blood dynamics factor result the cardiac muscle plump adjustment, research myocardial cell's biological mechanics, perishes, the acceptor to decline, the ischemia and processing, the signal passage weakly, to affects carries on screening in heart's new medicine and carries on the appraisal to its security, as well as withdraws the valuable biotic factor from the raise myocardial cell, becomes the myocardial cell with the medicine induction stem cell differentiation to make the masculine comparison and so on.The Chinese medicine traditional Chinese medicine receives the academic circle more and more diabetes heart disease's treatment the value, the traditional Chinese medicine prescription by its remarkable curative effect, the inexpensive cost, the few side effect depth is favored the patient. Take Professor Xiong Man Qi as head's research topic-based group in the1980s regarding was mad that cloudy two empty, the ecchymosis hindered for the pathogenesis, the peach seed received by "Typhoid" was mad that Tang Weide the side, developed Cheng Yuannei the preparation-to fall the sugar three yellow pieces, has made the big progress in cl inical and the mechanism research aspect, this experiment further discussed in various research foundation falls sugar three Huang Pian in the molecular cell level biology mechanism. Second part of experimental study goal:The observation falls sugar three Huang Pian to the high sugar induction under the myocardial cell weak perishing rate influence, the observation myocardial cell TGF-β1expression, further falls the sugar three Huang Pian action mechanism from the signal passage angle discussion. Method:1. the extraction contains the medicine blood serum. Ordinary level male New Zealand rabbit8, divide into normal group2stochastically, falls sugar three Huang Piandi monitoring team2, monitoring team2, high monitoring team2, the ordinary feed compatibility feeds for3days, falls sugar three Huang Piandi the monitoring team, gives0.75g/kg to fill the stomach; The monitoring team gives1.5g/kg to fill the stomach; The high monitoring team gives3g/kg to fill the stomach, the normal group pours into the isometric distilled water. Fills the clothing1week later, fasts12h, the abdomen aorta picks the blood, the extraction contains the medicine blood serum.-70℃the refrigerator saves frozen.2. myocardial cell raise and appraisal. The Wistar male gender is born3day of young mouse, takes the heart organization, the0.25%EDTA digestion over night, after15h, gives the isometric to cultivate stops digesting, gives the collagen digestive ferment ⅡThe digestion, separates fully the myocardial cell and the red blood cell, according to5X105/mL moves to12cell culture board the myocardial cell. Enters37℃the carbon dioxide incubator raise. after24h, obviously the myocardial cell beats voluntarily, and grows Large expanse, gives the Desmin immunity group appraisal, in the1:100Desmin group cytoblastema has the yellowish brown pellet masculine cell to occupy the total cell several about85%, the phosphoric acid buffer solution (PBS) the negative control group has not seen the masculine cell, then success raise myocardial cell.3. the class type cell meter examination cell perishes weakly.700ul including glucose (30mmol/L) all cultivates joins separately falls the sugar three yellow piece of low, middle and high monitoring teams and the model group, gives the different dosage to fall sugar three yellow piece of blood serum300ul separately again, the model group and Gao Tangzu joins all cultivates, after the intervention, after entering the carbon dioxide incubator raises48h, flows the type cell meter to examine each group of myocardial cell weak perishing rate.4. ELISA examination myocardial cell TGF-β1content. Perishes weakly after the examination cell the intervention myocardial cell supernate offcenter, is accepted after passing an examination clear, examines each group with the ELISA law the OD value, and according to the specification curve, calculates each group TGF-β1content. Finally:Flows type cell meter observation myocardial cell weak perishing rate, the normal group and the model group compare have the remarkable difference (p<0.05), falls the sugar during three yellow piece of low, middle and high groups the comparison to have the significance difference (p<0.05), and his/her myocardial cell weak perishing rate increases along with the dosage reduces, falls sugar three Huang Pianzu and the model group statistics difference (p<0.05), explained that falls the sugar three yellow pieces to be possible to suppress the high sugar induction the myocardial cell to perish weakly, and increases along with the medicine dosage, it suppresses the weak perishing rate degree to be more obvious; The ELISA examination myocardial cell TGF-β1shows falls sugar three Huang Piandi the monitoring team and the high monitoring team non-statistics difference (p>0.05), the monitoring team and the high dose, the low monitoring team have statistics difference (p<0.05), simultaneously lowers the monitoring team, the high monitoring team and the model group compares the non-statistics significance (p>0.05), the monitoring team and the model group compare have statistics significance (p<0.05), explained that falls sugar three Huang Piandi, the high dose and not obviously affects regarding the young mouse myocardial cell TGF-β1expression, but the dosage falls sugar three Huang Pianzu to be possible obviously to decline TGF-β1expression. Conclusion:1. the traditional Chinese medicine falls sugar three Huang Pianke to improve the high sugar induction the myocardial cell to perish weakly effectively, increases along with the density, suppresses the weak perishing rate degree to be stronger.2. the traditional Chinese medicine falls in sugar three Huang Pian the dosage to be possible remarkable under to raise the sugar induction the myocardial cell TGF-β1expression, its mechanism is possible and the upward is the membrane cell's GLUT1expression, subsequently activates protein kinase C (PKC) the amino sugar circuit to concern.