| Part one:Literature SummaryFatigue is a protective reaction when the body's exercise load is enough, fatigue is a physiological phenomenon. Exercise-induced fatigue are defined as a phenomenon of reduced motor ability caused by failure of maintaining the normal function of the organism at a certain level and/or at the projected intensity during the physiological process of the organism. The study on occurrence mechanism of exercise-induced fatigue including exhaustion theory, blocking-up hypothesis,mutation theory,free radical theory, etcExercise-induced fatigue pertains to the category of excessive physical labor in the rangeof overstrain according to traditional Chinese medicine. According to the visceral manifestation theory, the occurrence and development of exercise-induced fatigue approximately is related to viscera. In the aspects of five viscera, Traditional Chinese Medicine considers that "the kidney stores the essence", kidney in charge of bone and Marrow, we considerer kidney as the congenital foundationg, the origination source of power. The deficiency of kidney essence can influence the bone and muscle development which could easy cause the exercise-induced fatigue. Traditional Chinese Medicine considers that "The spleen provides the materiel basis of the acquired constitution, is the source of growth and development", and occupies an important position in the course of life activity.Spleen controls the muscle and four limbs which are related to Sports. If the spleen get hurts, the muscle became dysplasia, then the deficiency of strength, liver storing blood, when people doing sports, large blood stream to skeletal muscle, so we need liver regulate the flow of blood, to support the movement of limbs, liver controls the activity of the tendon, if the liver function is normal, the tendon and joints get nourished by the abundant liver blood, liver could make the sports powerful, smoothly, and persistently, the deficiency of liver blood induce the malnurition of tendon, so the movement will get slow and will to be exercise-induced fatigue quickly. From the theory of "heart governs the blood, the heart storing spirit", heart is closely related to the athletes'blood circulation will and character. We also considered that from the view of TCM, exercise-induced fatigue is not only asthenia syndrome but also excess syndrome, meanwhile exercise-induced fatigue is a disease with combination of excess and asthenia syndromes.Part two:Study target and ContentGuangdong herbal tea is widely consumed in Guangdong as traditional health drinks, with the function "heat-clearing and detoxifying, preventing suffer from excessive internal heat".With the improvement of living standards in recent years, more and more people are concerned about health care and health, as a class of herbal-based drinks tea not only more and more of the Guangdong residents love, but also herbal tea has been developed throughout all China. But the relatively ambiguous conceptof heat-clearing and detoxifying, preventing suffer from excessive internal heat is lack of precise definition.Based on Specialists-recommending, acupuncture is a good method to dispel exercise-induced fatigue.According to Traditional Chinese Medicine theory, this research select Wang Laoji herbal tea and Deng Lao herbal tea as the represents of Guangdong herbal, to compare the effect of Guangdong herbal and acupuncture on exercise-induced fatigue, from a brand-new perspective of pathology,antioxidation, biochemistry which has provided further theoritcal support and scientific data to elucidate this concept, as well as to promote and develop herbal tea. By the research, we want to make beneficial study and attempt of preventing exercise-induced fatigue.Content1. The effect of exercise^Guangdong herbal tea,acupuncture on the daily behavior and symptoms of rat.2. The influence of different kinds herbal tea supplement on the index of skeletal muscle, the function of kidney and liver, biochemorphology, biochemistry of rats. 3. The effect of acupuncture points on the index of skeletal muscle,the function of kidney and liver, biochemorphology, biochemistry of rats.4. To investigate the possible mechanism of herbal tea and acupuncture on exercise-induced fatigue.Part three:Research method and ConclusionMethodThere are all together72male SD rats, they are randomly divided into six groups:control group, fatigue treadmill training group, Wang Laoji herbal tea group, Deng Lao herbal tea group, pocari group, acupuncture group, After one week of natural feeding, except for the control, the other five group received2weeks training on treadmill, the training formula was based on the theory of Bedford, six times one week, last for two weeks, the acupuncture group accepted acupuncture treatment at the same time, Zhongwan and Zusanli were selected for acupuncture. The three beverage group accepted gastric perfusion with beverage. Draw material like hippocampus,skeletal muscle, kidney,liver,serum after exhausting exercise. Related indexes were detected after training; content of MDA in hippocampus and skeletal muscle, the T-AOC activity of hippocampus and skeletal muscle,content of glucose,content of blood ammonia,BUN,blood lactate,free calmodulin of skeletal muscles, CR,activities of acetylcholinesterase,expression of the caspase-3in hippocampus and skeletal muscle. Kidney,live and hippocampus were obtained from n microscopic examination.Conclusion1. Compared with the control group, by the two weeks treadmill training, the exercise-induced fatigue rat's contents of MDA in skeletal muscles,blood ammonia,blood lactate,free calmodulin of skeletal muscles,expression of the caspase-3in hippocampus and skeletal muscle increased significantly, the T-AOC markedly decreased. The index of peripheral and central have changed.It suggest that the exercise-induced model was successed. The symptom of fatigue treadmill training group suggest that, exercise-induced fatigue is not only asthenia syndrome but also excess syndrome, meanwhile is a disease with combination of excess and asthenia syndromes.2. Our experiment suggest that, Wang Laoji herbal tea and Deng Lao herbal tea could effectly reduce the content of MDA in skeletal muscle,increase in skeletal muscle of the total antioxidant capacity. They could increase the total antioxidant capacity of hippocampus in rat, reduce the content of MDA in skeletal muscle. We deduced that the function " heat-clearing and detoxifying, preventing suffer from excessive internal heat" of Guangdong herbal tea is closely related to the antioxidant capacity.3. Wang Laoji herbal tea and Deng Lao herbal tea could supply the energy material during exercise, maintain the level of blood glucose, ruduce the injury of liver and the membrane of hearts, reduce the level of AST.4. Acupuncture Zusanli, could prevent and treat exercise-induced fatigue in many steps and aspects. In the aspect of skeletal muscle:It could reduce the content of MDA inskeletal muscle, increase the total antioxidant capacity of skeletal muscle, reduced the content of calcium. In the aspect of the function of liver and kidney.-It could increase the content of liver glycogen, muscle glycogen, reduced the BUN. In the aspect of central nervous, the benign regulation effect of the protect mechanism of acupuncture on the neuron of exercise-induced rat might be reduce the expression of the caspase-3, prevent the activation of caspase-3, finally reach the purpose of anti-apoptosis, have protect the brain tissue after intensive exercise,but the related mechanism is not clear, it still need further study.Part four:Innovation point,Deficiency and Prospect Innovation Point1. In theory, by reviewing the related literatures for the review, combined with the observation of the symptoms through experiments, the author raised this noval hypothesis that:exercise-induced fatigue is a disease with combination of excess and asthenia syndromes.2. In terms of application, this research made a very comprehensive comparison of the impact of two different means of Traditional Chinese Medicine: Guangdong herbal tea and acupuncture on exercise-induced fatigue from all different perspectives.So this not only gives us inspiration on how to make the prevention and treatment of sports fatigue with Chinese medicine, but also would have profound influence for clinical and research evaluation, intervention indicators and tools.3. In terms of achivements, until now, no relevant research had conducted before neither in China nor abroad. This study firstly made application of Guangdong herbal tea to interfere with the exercise-induced fatigue. And, this research find the clues under the herbal tea "heat-clearing and detoxifying, preventing suffer from excessive internal heat " effect and antioxidant. By using the update techniques and methods of modern medicine, this research laid a certain foundation for the further study of Guangdong herbal tea.Deficiency and Prospect1. It is essential to further perfect the Establishment and assessment of rats model, and further study should made on:whether this treadmill has completely imitate the traning state of the atheletes. What kind of index shoude be abtained to evaluate the central nervous fatigue. Whether it is reasonable to use the "TCM diagnostic methods" as the standardizedi ndex for exercise-induced fatigue.2. Limited by time and funds, this experiment preliminary confirmed the effects of exercise and acupuncture on the expression of apoptosis Caspase-3, its mechanisms and pathways still needs further research, the mechanisms and pathways of the movements to exercise on apoptosis in skel etal muscle and brain cells apoptosis.3. In the aspect of central nervous, acupuncture havethe benign regulation effect of the protect mechanism on the neuron of exercise-induced rat might be reduce the expression of the caspase-3, prevent the activation of caspase-3, finally reach the purpose of anti-apoptosis, have protect the brain tissue after intensive exercise, but the related mechanism is not clear, it still need further study.4. This preliminary results indicated that Wang Laoji herbal tea and D eng Lao herbal tea could improve the state of exercise-induced fatigue, the function "heat-clearing and detoxifying, preventing suffer from exces sive internal heat "of Guangdong herbal tea is closely related to the anti oxidant capacity. We should discuss which effective components in the her bal tea in our further research... |
PDF Full Text Request