The Intervention Effects About Huoxue Herbs And Lishui Herbs On The Changes Of Brain Water Content In Intracerebral Hemorrhage(ICH) Rats

ObjectiveTo investigate possible mechanism of Huoxue Herbs and Lishui Herbs on the changesof brain water content in intracerebral hemorrhage(ICH) rats.Method1. SD rats were randomly divided into the following groups:the sham operatedgroup,model group,Huoxue group and Lishui group,36rats in each group．Each groupwas divided into three time phase point of1day,3days,5days again,12rats in each time.Experimental intracerebral hemorrhage rat model was established by injection autologousblood；2. The brain water content was determined by wet-dry weight；3. The pathological changes around perihematoma were observed by HE；4. The expression of AQP4and PAR-1were obvserved by immunohistochemitry,Western blot and real-time PCR at protein and mRNA levels respetively；5.The expression of NF-κB, TNF-α and IL-1β were obvserved byimmunohistochemitry and real-time PCR at protein and mRNA levels respetively．Result1. Compared with the sham group, the expression of AQP4,PAR-1NF-κB,TNF-αand IL-1β increased significantly at protein and mRNA levels(P<0.01)in the model group,the brain water content showed the same result； 2. Compared with the model group,the expression of AQP4,PAR-1,TNF-α andIL-1β decreased significantly at protein and mRNA levels (P<0.01)in Huoxue group andLishui group,the brain water content showed the same result；the expression of NF-κBdecreased obviously in Huoxue group,but have no difference in Lishui group；3. There were also significances in statistics on the brain water content and theexpression of AQP4,PAR-1,NF-κB,TNF-α and IL-1β between the Huoxue group andLishui group；4. In the model group, correlation analysis showed that there were positivecorrelations between the expression of AQP4and the water content, between theexpression of AQP4and PAR-1and between the expression of PAR-1and NF-κB,TNF-αand IL-1β respectivedly；5. Significant pathological changes were happened around perihematoma,while theywere improved in Huoxue group and Lishui group respectivedly.Conclusions1. The road of Thromb-PAR-1—inflammation reaction perhaps a part of upstreamsignaling pathways of AQP4；that is to say,probably through the inflammation reactionmediated by Thromb-PAR-1, the expression of AQP4around perihematoma wasupregulated,this resulted the edema of ICH；2. Huoxue Herbs and Lishui Herbs can decrease the edema of ICH,the probablelymechanism was that it could regulate the expression of related proteins and cytokinesaround perihematoma,but herbs of different functions have different effects,targets sandintensity.