Pharmacodynamic Material Basis And Part Of The Mechanism Of Traditional Chinese Medicine Capsules Kuntai Prevention Female Menopause Syndrome

With the Instruction of traditional Chinese medicine, make use of the research method which is in close cooperation of pharmacology and chemistry, Study active substance elements and pharmacological operational mechanism of the effective prescription-herb, QUNTAI Capsule, which is used to prevention and cure Female climacteric state Syndrome.To sum up effective prescription-herb of near 5 years which is used to prevention and cure Female climacteric state Syndrome and discuss their operational mechanism. According to available research results of Female climacteric state Syndrome witch is treated by west medicine or traditional Chinese medicine, we put forward thai Onset of Female climacteric state Syndrome is in close correlation of disorder of Qi transformation of spleen-stomach and the body. So we study out therapeutic principle of regulating functional activities of Qi and tonifying Qi transformation. According to the research results of ShengMa and ChaiHu, make the compound prescription (QUNTAI Capsule) which is made of them to prevention and cure Female climacteric state Syndrome. And we put forward firstly that Qi transformation disorder of liver, spleen and stomach is the important pathogenesis of Female climacteric state Syndrome and we study operational mechanism of compound prescription (QUNTAI Capsule) which is made of ShengMa and ChaiHu.Guided by medicine effect marker, separate and purify 3 elements(A, B, C) from QUNTAI Capsule, screen them directionally to confirm the substance basis.The results hint that sample A of QUNTAI Capsule is able to rise wet weigh index of womb and ovary of impuberism mouse significantly, rise Opening rate of impuberism Mouse vagina, sample A of QUNTAI Capsule has similar action to estrogen significantly and its action is stronger than sample A and B of QUNTAI Capsule. So sample A of QUNTAI Capsule is the medicine effect substance base ofQUNTAI Capsule.Select 14~ 16 months age rat model, castrated rat model, feed them with effectivepart A of QUNTAI Capsule 15 days continuity. Discuss the prevention and cure Action and its mechanism of QUNTAI Capsule for Female climacteric state Syndrome by diecover the histology changes of ovary and pituitary gland and influence of reproduction, 'endocrine and womb ER. The results show that effective part A of QUNTAI Capsule from the therapeutic principle of regulating liver and spleen and tonifying Qi transformation has several actions: (1) increase cornification index of vagina and wet weigh index of womb, Adjust Blood sex hormone Level, increase granular cell layer depth of ovary, accelerate growth of ovarian follicle; (2) adjust Cell density and Cell classification of Pituitary anterior lobe, promote blood vessel regeneration of Pituitary anterior lobe and ovary, expand blood vessel, ameliorate Local blood circulation of pituitary gland and ovary; (3)up-regulate ER Content of womb nucleus and tissue of climacteric Rat and castrated Rat. Comparing with nilestriol, up-regulation Action for womb cell nucleus receptor Quantity of QUNTAI Capsule is stronger, QUNTAI Capsule has the di-direction regulating action for E2 level in blood serum, and its stimulation formucous membrane is slenderer than nilestriol's.Results of medicine effect of QUNTAI Capsule conform that therapeutic principle of regulating functional activities of liver and spleen Qi and tonifying Qi transformation is able to prevention and cure Female climacteric state Syndrome very well. And we put forward a modern screen flow for Chinese herb, effective prescription-herb - separate and purify - screening of medicine effect - effective elements - action mechanism - making new medicine, this is a effective method toscreen effective substance and create modern Chinese medicine.