Mal Gene Changes In Human Esophageal Carcinoma Rna In Situ Hybridization Study

Malignant tumor is a series of diseases affecting human health severely. The incidence of the disease in our country is as high as 1.6 million, and 1.3million of patients will die every year. In a recent survey of cancer, mortality of esophageal cancer ranked the 4th in cities and the 3rd in rural areas during 1990-1992.Esophageal cancer is associated with many factors such as imbalance of diet, lack of some trace elements and habit. All of those are important in the etiology of esophageal cancer.A lot of research have been conducted on the molecular mechanism of esophageal cancer in recent years. They mainly focused on the role of some oncogenes and antioncogenes, disorderly regulation of some genes controlling growth and differentiation . Xu identified a cDNA fragment which is the 3'end of MAL gene after GeneBank search. MAL gene was initially identified by Alonso and Weissman in their study on differentiation of T-cells in 1987.The gene expresses in the mediate and late stage of T-lymphocyte. MAL was mapped to the proximal long arm of human chromosome2(q13) with four exons ,1051bp cDNA,462bp coding sequence, encoding a high hydrophobic contains four potential transmambrane, molecular 16.7kD protein. It is believed that MAL gene associated with differentiation. Northern blot of the gene indicated that MAL highly express in esophageal epithelial but not in esophageal cancer tissues. According to the results, we detected the expression in esophageal cancer and adjacent esophageal epithelial by nonradioactive In Situ Hybridization. In order to avoid nonspecific signals, we transcribed DIG-labeled sense/anti-sense cRNA probe in vitro according to coding sequence by SP₆ and T₇ polymerase respectively. The results suggested that...