Rheumatoid Arthritis, Ankylosing Spondylitis And Osteoarthritis Synovium Into Fibroblast-like Cell Proliferation And Differentiation In Vitro Studies

OBJECTIVE: Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease that mainly affects multiple synovial joints. Synovitis may be present in RA. Inflammation is accompanied by an influx of immune competent cells and by aberrant proliferation of resident fibroblast-like synovial cells (FLS) . Accretion of FLS is responsible for progressive joint destruction.T-614 [N-(3-formylamino- 4-oxo-6-phenoxy-4H-chromen-7-yl) methanesulfonamide] is known as cyclooxygenase-2(COX-2) inhibitor and total glucosides of paeony (TGP) are active compounds extracted from the roots of Paeonia lactiflora Pall, both are efficient in anti-inflammation and immunoregulation in arthritis via various pathways. The tumor necrosis factor-alpha (TNF-α) inhibitor thalidomide is known to be a potential modulator of host immunity, a potential treatment for autoimmune disorders such as rheumatoid arthritis (RA).All trans retinoid acid (ATRA) , 1,25 (OH)₂D₃ and desamethasone are known as inducer for cell differentiation and inhibitor to fibroblast cells proliferatiation. The effect on FLS proliferatiation and apoptosis is still unclear.In this study, we investigate the proliferation characteristics of FLS in RA and OA patients in vitro and the mechanism of the immunosuppressive effect of thalidomide, TGP , T-614, ATRA, 1,25 (OH)₂D₃ and desamethasone on them.